Acosta-Virgen Karla, González-Conchillos Hugo David, Vallejo-Flores Gabriela, Salazar-Villatoro Lizbeth Iliana, Guerrero-Sánchez Ernesto, Martínez-Palomo Adolfo, Espinosa-Cantellano Martha
Departamento de Infectómica y Patogénesis Molecular, Center for Research and Advanced Studies, Mexico City, Mexico.
Department of Molecular Biology, Max Planck Institute for Infection Biology, Berlin, Germany.
PLoS One. 2025 Mar 13;20(3):e0319701. doi: 10.1371/journal.pone.0319701. eCollection 2025.
Three-dimensional cultures are powerful tools to recapitulate animal and human tissues. Under the influence of specific growth factors, adult stem cells differentiate and organize into 3D cultures named organoids. The molecular phenotyping of these structures is an essential step for validating an organoid model. However, the limited number of organoids generated in culture yields very low amounts of genetic material, making phenotyping difficult. Recently, digital PCR (dPCR) techniques have become available for the highly sensitive detection of genetic material at low concentrations. The aim of this work was to apply dPCR to the identification of the various cell populations expected to be present in murine duodenal organoids. Results show the potential use of dPCR as a genetic characterization tool for organoids.
三维培养是模拟动物和人体组织的有力工具。在特定生长因子的影响下，成体干细胞分化并组织成名为类器官的三维培养物。这些结构的分子表型分析是验证类器官模型的关键步骤。然而，培养中产生的类器官数量有限，导致遗传物质产量极低，使得表型分析变得困难。近年来，数字PCR（dPCR）技术已可用于低浓度遗传物质的高灵敏度检测。这项工作的目的是应用dPCR来鉴定预期存在于小鼠十二指肠类器官中的各种细胞群。结果表明dPCR作为类器官遗传特征分析工具的潜在用途。
