Ahlstedt S, Björkstén B, Borwell J, Hammarström I, Nybacka J O
Int Arch Allergy Appl Immunol. 1985;77(1-2):238-40. doi: 10.1159/000233798.
Maturation of basophils/mast cells as well as formation of IgE antibodies are parts of the immune response to antigens. We have analyzed these important constituents of the allergic inflammatory response in vitro, cultivating human peripheral blood cells from allergic and normal individuals. Mesenteric lymph nodes were also cultured from two normals. In liquid cultures of peripheral blood cells, the number of small basophils initially present decreased and large basophilic cells appeared over a 2-week period. In normals, the maturation of large basophils from precursor cells could be stimulated with supernatants from peripheral blood cell cultures from atopic individuals stimulated with the relevant allergen. The IgE levels declined in cultures of peripheral blood, whereas the IgE antibodies in cultures of cells from a mesenteric lymph node from one of the nonallergic individuals increased during the 2-week period. In separate cultures, the IgE formation could not be reproducibly stimulated by antigen or mitogen. The study shows the possibility to stimulate the maturation of basophil/mast cell precursor cells present in the blood of allergic and healthy individuals with antigen-induced factors. In contrast, the IgE formation by blood cells was difficult to stimulate.
嗜碱性粒细胞/肥大细胞的成熟以及IgE抗体的形成是对抗抗原免疫反应的一部分。我们通过培养过敏个体和正常个体的人外周血细胞，在体外分析了过敏炎症反应的这些重要组成部分。还从两名正常人身上培养了肠系膜淋巴结。在外周血细胞的液体培养中，最初存在的小嗜碱性粒细胞数量减少，大嗜碱性细胞在2周内出现。在正常人中，来自特应性个体的外周血细胞培养上清液，用相关过敏原刺激后，可刺激前体细胞向大嗜碱性粒细胞的成熟。外周血培养物中的IgE水平下降，而来自一名非过敏个体的肠系膜淋巴结细胞培养物中的IgE抗体在2周内增加。在单独的培养中，抗原或有丝分裂原无法重复刺激IgE的形成。该研究表明，用抗原诱导因子刺激过敏个体和健康个体血液中存在的嗜碱性粒细胞/肥大细胞前体细胞成熟是可能的。相比之下，血细胞形成IgE则难以被刺激。
