Nagata K, Okada M
J Biochem. 1985 Feb;97(2):501-7. doi: 10.1093/oxfordjournals.jbchem.a135084.
The properties of crude and purified mitochondrial aspartate aminotransferase preparations from pyridoxine-deficient and control rat livers were compared. The preparations from the two sources showed very similar behaviors on heat treatment, electrophoresis and chromatofocusing, and had similar molecular weights, but their visible absorption spectra and circular dichroism properties were different. These results suggest that mitochondrial aspartate aminotransferase from pyridoxine-deficient and control rat livers have very similar properties, but differ somewhat in conformation in the region of the pyridoxal phosphate binding site.
对来自吡哆醇缺乏和对照大鼠肝脏的粗制和纯化线粒体天冬氨酸氨基转移酶制剂的性质进行了比较。来自这两种来源的制剂在热处理、电泳和色谱聚焦方面表现出非常相似的行为，并且具有相似的分子量，但它们的可见吸收光谱和圆二色性性质不同。这些结果表明，来自吡哆醇缺乏和对照大鼠肝脏的线粒体天冬氨酸氨基转移酶具有非常相似的性质，但在磷酸吡哆醛结合位点区域的构象略有不同。
