用吡哆醇治疗对吡哆醇缺乏大鼠组织中天冬氨酸转氨酶活性的影响。
Effect of treatment with pyridoxine on aspartate aminotransferase activities in pyridoxine-deficient rat tissues.
作者信息
Okada M, Kondo T
出版信息
J Nutr Sci Vitaminol (Tokyo). 1982 Apr;28(2):69-75. doi: 10.3177/jnsv.28.69.
In rat liver, 90% of the aspartate aminotransferase is present as the holoenzyme. In pyridoxine deficiency, the ratio of holoenzyme activity to total activity is markedly reduced, but after pyridoxine injection it was found to rapidly increase, although the total enzyme activity remained low for a few days. The activities of aspartate aminotransferase isozymes in pyridoxine-deficient rat tissues and the effect of pyridoxine treatment on their activities were examined. The intestinal enzyme activities of pyridoxine-deficient rats were readily reconstituted in the presence of pyridoxal phosphate in vitro, but the enzyme activities in liver and muscle in the deficient rats required several days for complete recovery, suggesting that active enzyme was synthesized de novo in these tissues.
在大鼠肝脏中，90%的天冬氨酸转氨酶以全酶形式存在。在维生素B6缺乏时，全酶活性与总活性的比值显著降低，但注射维生素B6后，发现该比值迅速升高，尽管总酶活性在几天内仍保持较低水平。研究了维生素B6缺乏的大鼠组织中天冬氨酸转氨酶同工酶的活性以及维生素B6治疗对其活性的影响。维生素B6缺乏大鼠的肠道酶活性在体外存在磷酸吡哆醛时很容易恢复，但缺乏大鼠肝脏和肌肉中的酶活性需要几天才能完全恢复，这表明这些组织中从头合成了活性酶。
Zotero 插件