Shibuya M, Nagata K, Okada M
J Biochem. 1982 Nov;92(5):1399-402. doi: 10.1093/oxfordjournals.jbchem.a134063.
The enzyme and antigen activities of aspartate aminotransferase [EC 2.6.1.1] isozymes in several tissues of rats fed on pyridoxine-deficient or control diet for 4 weeks were determined. The enzyme activities in liver (supernate, mitochondria), heart (supernate), brain (mitochondria), and muscle (mitochondria) preparations of the deficient rats were abnormally low, whereas those in other tissue preparations were similar to those of control rats. In liver and heart preparations, the antigen activities of the deficient rats were similar to those in controls, but in brain and muscle mitochondria they were abnormally low. These findings suggest that the activity and the amount of this enzyme are regulated in different ways in different tissues, and suggest that the coenzyme level affects metabolic regulation of the enzyme molecule.
测定了用缺乏维生素B6的饲料或对照饲料喂养4周的大鼠几种组织中天冬氨酸转氨酶[EC 2.6.1.1]同工酶的酶活性和抗原活性。缺乏维生素B6的大鼠肝脏（上清液、线粒体）、心脏（上清液）、脑（线粒体）和肌肉（线粒体）制剂中的酶活性异常低，而其他组织制剂中的酶活性与对照大鼠相似。在肝脏和心脏制剂中，缺乏维生素B6的大鼠的抗原活性与对照大鼠相似，但在脑和肌肉线粒体中，它们异常低。这些发现表明，该酶的活性和数量在不同组织中以不同方式受到调节，并表明辅酶水平影响酶分子的代谢调节。
