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可靠地大规模生产铃木果蝇(双翅目:果蝇科)的幼虫寄生蜂——基氏伽纳小蜂(膜翅目:褶翅小蜂科)。

Reliable mass production of Ganaspis kimorum (Hymenoptera: Figitidae), a larval parasitoid of Drosophila suzukii (Diptera: Drosophilidae).

作者信息

Huang Juan, Isaacs Rufus, Wilson Julianna K

机构信息

Department of Entomology, Michigan State University, East Lansing, MI, USA.

出版信息

J Insect Sci. 2025 Mar 14;25(2). doi: 10.1093/jisesa/ieaf024.

DOI:10.1093/jisesa/ieaf024
PMID:40085515
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11908422/
Abstract

Spotted wing drosophila, Drosophila suzukii (Matsumura), native to Asia, has become a significant threat to soft fruit crops globally. To develop a classical biological control program for this pest, the obligate parasitoid Ganaspis kimorum (Buffington) was approved in the United States for field release in 2021 as a biological control agent. However, challenges in mass production and maintenance of parasitoid colonies have been common. Here, we share improved methods and offer insights into mitigating issues that limit parasitoid production. Additionally, we present a modified rearing protocol using 2-l plastic containers to produce an average of 307 G. kimorum per container. This information is crucial for implementing successful classical biological control programs against spotted wing drosophila with this parasitoid.

摘要

铃木氏果蝇(Drosophila suzukii Matsumura)原产于亚洲,现已成为全球软果作物的重大威胁。为了针对这种害虫制定经典的生物防治方案,专性寄生蜂基氏加纳斯寄蝇(Ganaspis kimorum Buffington)于2021年在美国被批准作为生物防治剂进行田间释放。然而,寄生蜂种群的大规模生产和维持一直存在挑战。在此,我们分享改进方法,并就缓解限制寄生蜂生产的问题提供见解。此外,我们提出了一种改良的饲养方案,使用2升塑料容器,每个容器平均可生产307只基氏加纳斯寄蝇。这些信息对于利用这种寄生蜂实施针对铃木氏果蝇的成功经典生物防治方案至关重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec7f/11908422/3c7da43ed419/ieaf024_fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec7f/11908422/6d2795fba7d5/ieaf024_fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec7f/11908422/beafbda8c593/ieaf024_fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec7f/11908422/3c7da43ed419/ieaf024_fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec7f/11908422/6d2795fba7d5/ieaf024_fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec7f/11908422/beafbda8c593/ieaf024_fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec7f/11908422/3c7da43ed419/ieaf024_fig2.jpg

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本文引用的文献

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Methods for Rearing the Parasitoid Ganaspis brasiliensis, a Promising Biological Control Agent for the Invasive Drosophila suzukii.饲养寄生蜂 Ganaspis brasiliensis 的方法,该寄生蜂是入侵性果蝇 Drosophila suzukii 的有前途的生物防治剂。
J Vis Exp. 2022 Jun 2(184). doi: 10.3791/63898.
2
Drosophila suzukii (Diptera: Drosophilidae): A Decade of Research Towards a Sustainable Integrated Pest Management Program.铃木果蝇(双翅目:果蝇科):迈向可持续综合虫害管理计划的十年研究
J Econ Entomol. 2021 Oct 13;114(5):1950-1974. doi: 10.1093/jee/toab158.
3
Evidence for a cryptic parasitoid species reveals its suitability as a biological control agent.
证据表明一种隐匿寄生种适合作为生物防治剂。
Sci Rep. 2020 Nov 5;10(1):19096. doi: 10.1038/s41598-020-76180-5.
4
Host specificity of Asian parasitoids for potential classical biological control of .亚洲寄生蜂对潜在经典生物防治的寄主专一性。
J Pest Sci (2004). 2018;91(4):1241-1250. doi: 10.1007/s10340-018-1003-z. Epub 2018 Jun 18.
5
Reducing Drosophila suzukii emergence through inter-species competition.通过种间竞争减少果蝇的出现。
Pest Manag Sci. 2018 Jun;74(6):1466-1471. doi: 10.1002/ps.4836. Epub 2018 Feb 20.
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Insects. 2012 Aug 10;3(3):727-42. doi: 10.3390/insects3030727.
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