从一名首次发作的散发性精神分裂症男性青少年中衍生出人源诱导多能干细胞系。
Derivation of human-derived iPSC line from a male adolescent with first-episode of sporadic schizophrenia.
作者信息
Jiang Youhui, Zhou Chuqing, Zhao Jun, Ren Xinyi, Wang Qiang, Ni Peiyan, Li Tao
机构信息
The Mental Health Center and Psychiatric Laboratory, West China Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
Affiliated Mental Health Center & Hangzhou Seventh People's Hospital, Zhejiang University School of Medicine, Hangzhou 310013, People's Republic of China.
出版信息
Stem Cell Res. 2025 Jun;85:103694. doi: 10.1016/j.scr.2025.103694. Epub 2025 Mar 10.
Schizophrenia is considered to be a neurodevelopmental disorder with high heritability. In this study, peripheral blood mononuclear cells (PBMCs) were collected from a male adolescent diagnosed with first-episode of sporadic schizophrenia. Induced pluripotent stem cells (iPSCs) were generated by reprogramming using the factors OCT4, SOX2, NANOG, LIN28, c-MYC, KLF4, and SV40LT. The generated iPSC line was validated by karyotype analysis and expression of pluripotency markers. These iPSCs were capable of differentiating into derivatives of all three germ layers in vivo.
精神分裂症被认为是一种具有高遗传性的神经发育障碍。在本研究中，从一名被诊断为散发型精神分裂症首发的男性青少年中采集外周血单个核细胞（PBMC）。使用OCT4、SOX2、NANOG、LIN28、c-MYC、KLF4和SV40LT等因子通过重编程生成诱导多能干细胞（iPSC）。通过核型分析和多能性标志物的表达对所生成的iPSC系进行验证。这些iPSC能够在体内分化为所有三个胚层的衍生物。
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