从一名健康中国男性供体成功建立了诱导多能干细胞系（SDPHi005-A）。
An achievement has been made in establishing an induced pluripotent stem cell line (SDPHi005-A) from a healthy Chinese male donor.
作者信息
Shao Jing, Jin Yan, Shao Chunhong, Zhang Xiaoqiao, Yang Guang
机构信息
Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China.
Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China.
出版信息
Stem Cell Res. 2024 Jun;77:103393. doi: 10.1016/j.scr.2024.103393. Epub 2024 Mar 16.
Successfully transforming isolated peripheral blood mononuclear cells (PBMCs) from a healthy male into induced pluripotent stem cells (iPSCs) was achieved using non-integrating episomal vectors containing OCT4, SOX2, c-MYC, KLF4, and BCL-XL. The iPSCs exhibited a normal karyotype, expressed pluripotency markers, and demonstrated the ability to differentiate into three germ layers in vitro. This iPSC line may be employed for subsequent research purposes.
利用包含OCT4、SOX2、c-MYC、KLF4和BCL-XL的非整合型附加体载体，成功地将一名健康男性的分离外周血单个核细胞（PBMC）转化为诱导多能干细胞（iPSC）。这些iPSC表现出正常的核型，表达多能性标志物，并在体外显示出分化为三个胚层的能力。该iPSC系可用于后续研究目的。
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