Zmudzka Magdalena, Szramel Joanna, Karasinski Janusz, Nieckarz Zenon, Zoladz Jerzy A, Majerczak Joanna
Chair of Exercise Physiology and Muscle Bioenergetics, Faculty of Health Sciences, Jagiellonian University Medical College, Skawinska 8 Street, Krakow, 31-066, Poland.
Department of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Krakow, Poland.
Sci Rep. 2025 Mar 14;15(1):8848. doi: 10.1038/s41598-025-93176-1.
Fast locomotory muscles, which are responsible for generating the highest power outputs, are more vulnerable to aging than slow muscles. In this study, we aimed to evaluate the impact of middle age and voluntary physical activity on capillarization and angiogenic potential in fast locomotory muscles. Middle-aged (M-group) and young (Y-group) wild-type FVB female mice were randomly assigned to either the sedentary or trained group undergoing 8-week spontaneous wheel running (8-sWR). Capillary density (assessed via immunohistochemical capillary staining and Western immunoblotting) of the fast locomotory muscles in the M-group (15-months old) was not significantly different compared to the Y-group (4-months old). Nevertheless, the expression of key pro-angiogenic genes in the fast muscle of the M-group was lower than that in the fast muscle of Y-group. 8-sWR had no impact on muscle capillarization; however, it increased fast muscle Vegfa expression in both the M and Y groups. We concluded that although fast muscle capillarization is still preserved in middle age, nevertheless the angiogenic potential (at least at the level of gene expression) is significantly reduced at this stage of aging. Moderate-intensity voluntary physical activity had no effect on capillary density, but it increased the angiogenic potential of the fast muscle.
负责产生最高功率输出的快速运动肌肉比慢速肌肉更容易受到衰老的影响。在本研究中，我们旨在评估中年和自愿体育活动对快速运动肌肉中毛细血管化和血管生成潜力的影响。将中年（M组）和年轻（Y组）野生型FVB雌性小鼠随机分为久坐组或接受8周自发轮转跑步（8-sWR）的训练组。M组（15个月大）快速运动肌肉的毛细血管密度（通过免疫组织化学毛细血管染色和Western免疫印迹评估）与Y组（4个月大）相比无显著差异。然而，M组快速肌肉中关键促血管生成基因的表达低于Y组快速肌肉中的表达。8-sWR对肌肉毛细血管化没有影响；然而，它增加了M组和Y组快速肌肉中Vegfa的表达。我们得出结论，尽管中年时快速肌肉的毛细血管化仍然保持，但在这个衰老阶段血管生成潜力（至少在基因表达水平）显著降低。中等强度的自愿体育活动对毛细血管密度没有影响，但它增加了快速肌肉的血管生成潜力。
