Dörtbudak Muhammet Bahaeddin, Demircioğlu Muhammed, Kapucuk Fuat Serkan
Department of Pathology, Faculty of Veterinary Medicine, Harran University, Şanlıurfa, Turkey.
Department of Histology and Embryology, Institute of Health Sciences, Dicle University, Diyarbakir, Turkey.
Vet Med Sci. 2025 Mar;11(2):e70264. doi: 10.1002/vms3.70264.
Life-threatening sepsis with high mortality and morbidity is an important cause of acute kidney injury and myocardial dysfunction. In this study, we investigated the protective effect of Micromeria congesta (MC) against kidney and heart damage caused by lipopolysaccharide (LPS) used as a sepsis model. Control, LPS, LPS + 25 mg/kg MC and LPS + 50 mg/kg MC groups were established from rats for the study. After the experiment, kidney and heart tissues obtained from the rats were stained with hematoxylin-eosin for histopathologic examination. Immunohistochemical staining was performed to determine inflammation, apoptosis, oxidative stress and DNA damage. IL-2 for inflammation, CASP-3 for apoptosis, HSP-27 for oxidative stress and 8-OHdG for DNA damage were used for immunopathologic examination. Histopathologic examination showed that the lesions in the kidney and heart tissues in the LPS group decreased with increasing doses of MC. Immunohistochemical examination showed that the expression of IL-2, CASP-3, HSP-27 and 8-OHdG was severe in the LPS group, but the severity of expression in these tissues decreased with increasing doses of MC. As a result of the study, it was histopathologically determined that MC reduced LPS-induced kidney and heart tissue damage. In addition, MC was found to protect against LPS by reducing LPS-induced inflammation, apoptosis, oxidative stress and DNA damage in kidney and heart tissue. In conclusion, it was seen that MC was effective in sepsis damage. However, it was concluded that MC could be an alternative in drug strategies developed for sepsis treatment with studies in vivo including more analyses.
具有高死亡率和发病率的危及生命的脓毒症是急性肾损伤和心肌功能障碍的重要原因。在本研究中，我们研究了密花黄芩（MC）对由用作脓毒症模型的脂多糖（LPS）引起的肾脏和心脏损伤的保护作用。从大鼠中建立对照组、LPS组、LPS + 25 mg/kg MC组和LPS + 50 mg/kg MC组用于该研究。实验结束后，从大鼠获取的肾脏和心脏组织用苏木精-伊红染色进行组织病理学检查。进行免疫组织化学染色以确定炎症、凋亡、氧化应激和DNA损伤。使用IL-2检测炎症、CASP-3检测凋亡、HSP-27检测氧化应激、8-OHdG检测DNA损伤进行免疫病理学检查。组织病理学检查显示，LPS组肾脏和心脏组织中的病变随着MC剂量增加而减少。免疫组织化学检查显示，LPS组中IL-2、CASP-3、HSP-27和8-OHdG的表达严重，但这些组织中的表达严重程度随着MC剂量增加而降低。研究结果表明，组织病理学确定MC减轻了LPS诱导的肾脏和心脏组织损伤。此外，发现MC通过减少LPS诱导的肾脏和心脏组织中的炎症、凋亡、氧化应激和DNA损伤来保护免受LPS侵害。总之，可见MC在脓毒症损伤中有效。然而，通过包括更多分析的体内研究得出结论，MC可以成为脓毒症治疗所开发的药物策略中的一种替代选择。
