Rashed Hadeer Abd El-Hak, Elmajdoub Layla Omran, Fayad Eman, Almaaty Ali Hussein Abu
Zoology Department, Faculty of Science, Port Said University, Port Said, Egypt.
Zoology Department, Faculty of Science, Misurata University, Misurata, Libya.
Open Vet J. 2025 Jan;15(1):314-324. doi: 10.5455/OVJ.2025.v15.i1.30. Epub 2025 Jan 31.
Protists and helminths are considered the main organisms invading different cultures, especially aquatic organisms. Morphometric characteristics remain the most trustworthy descriptors of species identification or, more broadly, pattern recognition.
This study aimed to provide morphological descriptions of the most prevalent Protista and helminths found in various street wetlands in Port Said, Egypt.
This study collected three water samples per area each month. The samples were sent to the Parasitology Laboratory at Port Said University, Egypt. The samples were analyzed in two ways according to organism size. Large helminths were observed within 24 hours using a microscope, and their movements were recorded. Protists and helminth eggs were isolated by filtering water through a 0.45 μm cellulose acetate filter, followed by centrifugation. The isolated organisms were counted and imaged.
Nine dominant organisms were identified in the collected samples, including five parasitic species ( sp., , sp., sp., and sp.) and four free-living species ( sp., sp., sp., and sp.). sp. was the most abundant parasite (17.6%), exhibiting a cylindrical, elongated shape (97.3-139 µm in length, 30-48 µm in width). sp. were the free-living organisms (17%), measuring 59.7-66.5 µm in length and 32.1-42.5 µm in width, featuring cirri and dark bristles. sp., with lengths between 161 and 256 µm, was spindle-shaped and had visible contractile vacuoles. trophozoites measured 60-107 µm in length and 46.5-83.3 µm in width, with a large posterior macronucleus. sp. larvae were about 700 µm long, with a distinct tail filament assisting their complex movement.
The street wetlands in the surveyed area contain various free-living and pathogenic taxa. There is a need to link the presence of these organisms to physicochemical analyses. Future studies should prioritize broader geographic sampling, the integration of molecular techniques, and the exploration of host-parasite relationships.
原生生物和蠕虫被认为是入侵不同养殖环境的主要生物，尤其是水生生物。形态测量特征仍然是物种鉴定或更广泛的模式识别中最可靠的描述符。
本研究旨在对埃及塞得港不同街道湿地中最常见的原生生物和蠕虫进行形态学描述。
本研究每月在每个区域采集三个水样。样本被送往埃及塞得港大学的寄生虫学实验室。根据生物体大小，样本采用两种方式进行分析。大型蠕虫在24小时内用显微镜观察，并记录其运动情况。原生生物和蠕虫卵通过0.45μm醋酸纤维素滤膜过滤水样后分离，然后进行离心。对分离出的生物体进行计数和成像。
在所采集的样本中鉴定出9种优势生物，包括5种寄生物种（种、种、种、种和种）和4种自由生活物种（种、种、种和种）。种是最丰富的寄生虫（17.6%），呈圆柱形、细长形（长97.3 - 139μm，宽30 - 48μm）。种是自由生活生物（17%），体长59.7 - 66.5μm，宽32.1 - 42.5μm，具有纤毛和深色刚毛。种体长在161至256μm之间，呈纺锤形，有可见的收缩泡。滋养体体长60 - 107μm，宽46.5 - 83.3μm，有一个大的后部大核。种幼虫长约700μm，有明显的尾丝辅助其复杂运动。
调查区域的街道湿地包含各种自由生活和致病类群。有必要将这些生物的存在与理化分析联系起来。未来的研究应优先进行更广泛的地理采样、整合分子技术以及探索宿主 - 寄生虫关系。
