Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.
Department of Medical Biology, Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic.
Emerg Microbes Infect. 2023 Dec;12(2):2270077. doi: 10.1080/22221751.2023.2270077. Epub 2023 Oct 27.
, a cosmopolitan gastrointestinal protist, is detected mainly in patients with clinical giardiasis in high-income countries. In contrast, there is very little information on the presence of in asymptomatic individuals. Therefore, the aim of this study was to determine the presence and prevalence of in gut-healthy volunteers in the Czech Republic and to perform a comparative evaluation of different diagnostic methods, since diagnostics is complicated. Our results confirmed that the qPCR method is the most sensitive method for detecting and revealed a prevalence of 7% (22/296) in asymptomatic individuals. In most cases, the colonization intensity ranged from 10-10. A conventional PCR protocol targeting the TPI gene was used to identify the assemblages. However, this protocol had limited sensitivity for amplification, effectively detecting colonization above an intensity of 10. In addition, was detected in 19% of the animals, which were closely associated with the study participants. However, due to methodological limitations, zoonotic transmission could not be clearly confirmed. Notably, contact with animals proved to be the only factor that had a significant impact on the incidence of in gut-healthy humans.
, 一种世界性的胃肠道原生动物，主要在高收入国家有临床贾第虫病的患者中检测到。相比之下，关于无症状个体中 的存在情况，信息非常有限。因此，本研究的目的是确定在捷克共和国的肠道健康志愿者中 是否存在，并对不同的诊断方法进行比较评估，因为 诊断很复杂。我们的结果证实，qPCR 方法是检测 的最敏感方法，并在无症状个体中发现了 7%（22/296）的流行率。在大多数情况下，定植强度范围在 10-10 之间。使用针对 TPI 基因的常规 PCR 方案来鉴定组合。然而，该方案对 的扩增灵敏度有限，仅能有效检测到定植强度高于 10 的情况。此外，还在 19%的动物中检测到了 ，这些动物与研究参与者密切相关。然而，由于方法学上的限制，无法明确确认人畜共患传播。值得注意的是，与动物接触被证明是影响肠道健康人群中 发生率的唯一显著因素。
