通过植入补片并添加间充质干细胞分泌组治疗的低雌激素动物模型中的组织完整性和愈合反应
Tissue integrity and healing response in hypoestrogenic animal model treated by mesh implantation with addition of mesenchymal stem cell secretome.
作者信息
Dewi Tri Isyani Tungga, Noviana Deni, Priosoeryanto Bambang Pontjo, Gunanti Gunanti, Subangkit Mawar
机构信息
Graduate Program in Animal Biomedical Sciences, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia.
Division of Surgery and Radiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia.
出版信息
Open Vet J. 2025 Jan;15(1):162-170. doi: 10.5455/OVJ.2025.v15.i1.15. Epub 2025 Jan 31.
BACKGROUND
Pelvic organ prolapse increases in prevalence and incidence in older women and hypoestrogenic conditions. Treatment with native tissue surgery has a fairly high recurrence rate. Mesh-augmented surgery is one of the most promising treatments for pelvic organ prolapse, with high effectiveness and low recurrence. Mesh-augmented surgery has a side effect of tissue erosion. The addition of secretome is expected to improve tissue integrity and reduce tissue erosion.
AIM
This study aimed to investigate the effect of adding the umbilical cord mesenchymal stem cell (UC-MSC) secretome on preventing tissue inflammatory responses, improving tissue integrity, and accelerating wound healing.
METHODS
A total of 32 female New Zealand white rabbit hypoestrogenic models were divided into two groups: the control group with normal mesh and the secretome group with artificial mesh. Hypoestrogenic models were created using the bilateral ovariectomy method. Mesh implantation was performed using a surgical method on hypoestrogenic rabbits. The animals were euthanized on days 7, 14, 28, and 90 after mesh implantation. Histopathology parameters included angiogenesis formation, fibroblast number, and collagen deposition area.
RESULT
The results of this study showed that the number of angiogenesis, fibroblast, and collagen deposition data in the secretome group showed higher significantly < 0.05) than those in the control group on days 7, 14, 28, and 90 post mesh implantation. The formation of new blood vessels (angiogenesis) in the secretome group demonstrated a mean value of 9.81 ± 2.2 compared to 0.37 ± 0.03 in the control. The number of fibroblasts in the secretome group averaged 151.00 ± 8.14, in contrast to 34.00 ± 13.37 in the control group. Collagen formation in the secretome group was also higher, with a mean value of 80.02 ± 6.71 compared to 59.49 ± 4.61 in the control group over 90 days of observation.
CONCLUSION
The administration of secretomes from UC-MSC improved tissue integrity and accelerated wound healing.
背景
盆腔器官脱垂在老年女性和雌激素缺乏状态下的患病率和发病率均有所增加。采用自体组织手术治疗的复发率相当高。网片增强手术是治疗盆腔器官脱垂最有前景的方法之一，具有高效性和低复发性。网片增强手术有组织侵蚀的副作用。添加分泌组有望改善组织完整性并减少组织侵蚀。
目的
本研究旨在探讨添加脐带间充质干细胞（UC-MSC）分泌组对预防组织炎症反应、改善组织完整性和加速伤口愈合的作用。
方法
将32只新西兰雌性白兔雌激素缺乏模型分为两组：正常网片对照组和人工网片分泌组。采用双侧卵巢切除术建立雌激素缺乏模型。对雌激素缺乏的兔子采用手术方法植入网片。在植入网片后的第7、14、28和90天对动物实施安乐死。组织病理学参数包括血管生成、成纤维细胞数量和胶原沉积面积。
结果
本研究结果显示，在植入网片后的第7、14、28和90天，分泌组的血管生成、成纤维细胞和胶原沉积数据显著高于对照组（P<0.05）。分泌组新血管形成（血管生成）的平均值为9.81±2.2，而对照组为0.37±0.03。分泌组的成纤维细胞数量平均为151.00±8.14，而对照组为34.00±13.37。在90天的观察期内，分泌组的胶原形成也更高，平均值为80.02±6.71，而对照组为59.49±4.61。
结论
给予UC-MSC分泌组可改善组织完整性并加速伤口愈合。
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