Senekovič Jan, Jelen Špela, Urbanek Krajnc Andreja
Faculty of Agriculture and Life Sciences, University of Maribor, Pivola 10, 2311 Hoče, Slovenia.
Plants (Basel). 2025 Mar 2;14(5):766. doi: 10.3390/plants14050766.
Phenolic compounds are strong antioxidant and antibacterial agents with great pharmacological, medicinal, nutritional, and industrial value. The potential of in stem node culture was investigated for the production of phenolic compounds and their elicitation with CuSO. Individual phenolic compounds in the samples were identified and quantified by using HPLC-PDA and HPLC-MS methods, while the content of total phenolic compounds, the content of total flavonoids, and the antioxidant activity of methanolic extracts were evaluated spectrophotometrically. The highest fresh and dry weights were obtained in plantlets treated with 0.5 mM CuSO for 42 days. The highest total phenolic content, total flavonoid content, and antioxidant activity of the extracts were determined in stem node cultures treated with 3 mM CuSO for 42 days. Under the latter conditions, the predominant representatives of the caffeoylquinic acids, -coumaric acid derivatives, kaempferol derivatives, and quercetin derivatives also achieved the highest content. The most abundant phenolic compound in all samples was the chlorogenic acid. The nodal culture of elicited with CuSO could potentially be used for the industrial production of phenolic compounds, especially caffeoylquinic acids. Moreover, considering the biochemical response to CuSO treatment and the ability to tolerate and accumulate copper, the potential application of in phytoremediation is also highlighted.
酚类化合物是具有强大抗氧化和抗菌作用的物质，具有很高的药理、药用、营养和工业价值。研究了[植物名称未给出]茎节培养物中酚类化合物的生产潜力及其对硫酸铜的诱导响应。采用高效液相色谱-光电二极管阵列检测法（HPLC-PDA）和高效液相色谱-质谱联用法（HPLC-MS）对样品中的单个酚类化合物进行鉴定和定量，同时采用分光光度法评估甲醇提取物中总酚化合物含量、总黄酮含量和抗氧化活性。用0.5 mM硫酸铜处理42天的幼苗获得了最高的鲜重和干重。用3 mM硫酸铜处理42天的茎节培养物中提取物的总酚含量、总黄酮含量和抗氧化活性最高。在后一种条件下，咖啡酰奎尼酸、对香豆酸衍生物、山奈酚衍生物和槲皮素衍生物的主要代表物含量也最高。所有样品中含量最丰富的酚类化合物是绿原酸。用硫酸铜诱导的[植物名称未给出]茎节培养物有可能用于酚类化合物的工业化生产，尤其是咖啡酰奎尼酸。此外，考虑到对硫酸铜处理的生化响应以及耐受和积累铜的能力，[植物名称未给出]在植物修复中的潜在应用也得到了凸显。
