Dinat Sarhana, Orchard Ané, Ncube Efficient, Chen Weiyang, Viljoen Alvaro, van Vuuren Sandy
Department of Pharmacy and Pharmacology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
Department of Pharmaceutical Sciences, Faculty of Sciences, Tshwane University of Technology, Pretoria, South Africa.
Chem Biodivers. 2025 Aug;22(8):e202403200. doi: 10.1002/cbdv.202403200. Epub 2025 Apr 1.
Propolis, a resin-like substance produced by bees, has previously shown antimicrobial activity against the ulcer-causing gut pathogen Helicobacter pylori. South African propolis, however, was yet to be investigated. This study aimed to investigate a comprehensive range of South African propolis for its antimicrobial activity against H. pylori and to investigate toxicity. A total of 51 samples were collected from around South Africa and comparatively analysed with three Brazilian samples. The antimicrobial broth microdilution assay was used to determine the minimum inhibitory concentration (MIC) of ethanolic propolis extracts against three clinical H. pylori strains. A total of 27 South African propolis extracts presented antimicrobial activity better than that of the Brazilian samples (MIC ≤ 0.51 mg/mL). Samples with the best anti-H. pylori activity were selected for chemical analysis using ultra-performance liquid chromatography-mass spectrometry. The compounds pinocembrin, 3-O-acetylpinobanksin, and pinobanksin were found to be the most abundant. All propolis extracts investigated in this study were considered non-toxic (mortality < 50%) when investigated using the brine shrimp lethality assay. This study demonstrates the in vitro potential of utilizing propolis for treating H. pylori infections and highlights the possible compounds responsible for the activity observed.
蜂胶是蜜蜂产生的一种树脂状物质，此前已显示出对引起溃疡的肠道病原体幽门螺杆菌具有抗菌活性。然而，南非蜂胶尚未得到研究。本研究旨在全面调查南非蜂胶对幽门螺杆菌的抗菌活性，并研究其毒性。从南非各地共收集了51个样本，并与3个巴西样本进行了比较分析。采用抗菌肉汤微量稀释法测定蜂胶乙醇提取物对3株临床幽门螺杆菌菌株的最低抑菌浓度（MIC）。共有27种南非蜂胶提取物表现出比巴西样本更好的抗菌活性（MIC≤0.51mg/mL）。选择具有最佳抗幽门螺杆菌活性的样本，采用超高效液相色谱-质谱联用仪进行化学分析。发现化合物松属素、3-O-乙酰基松属双黄酮和松属双黄酮含量最高。当使用卤虫致死试验进行研究时，本研究中调查的所有蜂胶提取物均被认为无毒（死亡率<50%）。本研究证明了利用蜂胶治疗幽门螺杆菌感染的体外潜力，并突出了可能导致观察到的活性的化合物。
