Shirani Asl Vida, Moghaddami Mohsen, Abbasi Sahar, Sohrabi Niloofar, Tandel Parisa, Tamaddon Gholamhossein
Division of Hematology and Blood Bank, Department of Laboratory Science, School of Paramedical Science, Shiraz University of Medical Sciences, Shiraz, 71439-14693, Iran.
Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Biochem Genet. 2025 Mar 18. doi: 10.1007/s10528-025-11062-x.
COVID-19, caused by SARS-CoV-2 infection, frequently induces thrombotic complications in affected individuals. P-selectin, a pivotal platelet marker, plays a central role in platelet-leukocyte aggregation, contributing to hemostasis and thrombosis. Additionally, D-dimer serves as an indicator of coagulation system activity, while miR-17-5p exhibits antiviral properties in respiratory infections. This study aimed to evaluate and compare the expression levels of D-dimer, P-selectin, and miR-17-5p in COVID-19 patients hospitalized in intensive care units (ICUs) and those in non-ICU wards. This cross-sectional study included 50 COVID-19 patients, divided into ICU and non-ICU groups. P-selectin expression was assessed using Flow cytometry, D-dimer levels were measured via chemiluminescence, and miR-17-5p expression was analyzed using quantitative real-time polymerase chain reaction (qRT-PCR). Our analysis revealed no significant difference in P-selectin expression levels between ICU and non-ICU patients (p = 0.1068). However, the expression levels of D-dimer and miR-17-5p were significantly elevated in ICU patients compared to non-ICU patients, with corresponding p-values of 0.032 and 0.0176, respectively. The heightened expression of D-dimer and miR-17-5p in ICU patients suggests their potential utility as predictive biomarkers for assessing the hemostatic status of COVID-19 patients.
由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染引起的2019冠状病毒病（COVID-19），常使受感染个体出现血栓形成并发症。P-选择素是一种关键的血小板标志物，在血小板-白细胞聚集中起核心作用，有助于止血和血栓形成。此外，D-二聚体是凝血系统活性的指标，而miR-17-5p在呼吸道感染中具有抗病毒特性。本研究旨在评估和比较入住重症监护病房（ICU）的COVID-19患者与入住非ICU病房的患者中D-二聚体、P-选择素和miR-17-5p的表达水平。这项横断面研究纳入了50例COVID-19患者，分为ICU组和非ICU组。采用流式细胞术评估P-选择素表达，通过化学发光法测定D-二聚体水平，使用定量实时聚合酶链反应（qRT-PCR）分析miR-17-5p表达。我们的分析显示，ICU患者和非ICU患者的P-选择素表达水平无显著差异（p = 0.1068）。然而，与非ICU患者相比，ICU患者的D-二聚体和miR-17-5p表达水平显著升高，相应的p值分别为0.032和0.0176。ICU患者中D-二聚体和miR-17-5p表达升高表明它们有可能作为评估COVID-19患者止血状态的预测生物标志物。
