Daguano Gastaldi Vinicius, Hindermann Martin, Wilke Justus B H, Ronnenberg Anja, Arinrad Sahab, Kraus Sabine, Wildenburg Anne-Fleur, Ntolkeras Antonios, Provost Micah J, Ye Liu, Curto Yasmina, Cortés-Silva Jonathan-Alexis, Butt Umer Javed, Nave Klaus-Armin, Miskowiak Kamilla Woznica, Ehrenreich Hannelore
Clinical Neuroscience, Max Planck Institute for Multidisciplinary Sciences, City Campus, 37075 Göttingen, Germany; Georg-August-University, Göttingen, Germany.
Clinical Neuroscience, Max Planck Institute for Multidisciplinary Sciences, City Campus, 37075 Göttingen, Germany.
Cell Rep Methods. 2025 Mar 24;5(3):101011. doi: 10.1016/j.crmeth.2025.101011. Epub 2025 Mar 17.
In rodent behavior research, observer-independent methods, such as the IntelliCage, enhance data collection in a social, and thus stress-reduced, environment. The IntelliCage system allows experimenters to create cognitive challenges for mice motivated by rewards. Given the extensive and diverse data from IntelliCage, there is a high demand for automated analysis. Here, we introduce IntelliR, a free and standardized pipeline for analyzing IntelliCage data, including a cognition index for performance comparison across challenges. IntelliR supports the automatic analysis of three challenges that cover spatial, episodic-like, and working memory with their reversal tests and can also be adapted for other designs. Results from three cohorts of adult female C57B6 mice showed improved task proficiency over time. To validate cognitive impairment detection, we used adult female NexCreERT2xRosa26-eGFP-DTA mice after neuron ablation in cortex and hippocampus, in which we observed reduced learning capabilities. IntelliR integrates easily into research, improving time management and reproducibility.
在啮齿动物行为研究中，诸如智能鼠笼（IntelliCage）等独立于观察者的方法，能在社交环境中增强数据收集，从而减轻压力。智能鼠笼系统使实验者能够为受奖励驱动的小鼠设置认知挑战。鉴于来自智能鼠笼的广泛多样的数据，对自动化分析的需求很高。在此，我们介绍IntelliR，这是一个用于分析智能鼠笼数据的免费且标准化的流程，包括一个用于跨挑战进行性能比较的认知指数。IntelliR支持对涵盖空间记忆、情景样记忆和工作记忆及其反转测试的三项挑战进行自动分析，并且还可适用于其他设计。来自三组成年雌性C57B6小鼠的结果显示，随着时间推移任务熟练度有所提高。为了验证认知障碍检测，我们在成年雌性NexCreERT2xRosa26-eGFP-DTA小鼠的皮层和海马体神经元消融后进行了实验，在这些小鼠中我们观察到学习能力下降。IntelliR易于融入研究，改善了时间管理和可重复性。
