Merrilees M J, Finlay G J
Lab Invest. 1985 Jul;53(1):30-6.
The human tumor cell lines, MM-96, FME, HCT-8, HT-29, MCF-7 and T-47D, in culture produced a factor or factors able to stimulate glycosaminoglycan (GAG) synthesis in human skin fibroblasts (HF). Conditioned growth media from the melanoma MM-96 and the colon carcinoma HT-29 produced a 10- and 8-fold stimulation of HF GAG synthesis, respectively, with an even larger stimulation of hyaluronic acid. Conditioned media from the melanoma FME and the breast carcinomas MCF-7 and T-47D stimulated GAG synthesis 2-fold, whereas media from the colon carcinoma HCT-8 gave a variable response often with no effect on GAG levels. Conditioned media from HF cultures had no effect on tumor cell GAG synthesis. Coculture of tumor cells and HF also resulted in increased GAG synthesis, and the degree of stimulation was similar to that with the conditioned media. Tumor cell-conditioned media were also effective in stimulating GAG synthesis by porcine smooth muscle cells and by chick embryo fibroblasts in culture, although the increase in GAG synthesis was much less than with HF cultures. These findings support the concept that the stromal desmoplasia characteristic of many growing and invasive tumors in vivo arises by tumor cell modulation of GAG synthesis by surrounding normal connective tissue cells.
培养中的人肿瘤细胞系MM - 96、FME、HCT - 8、HT - 29、MCF - 7和T - 47D产生了一种或多种能够刺激人皮肤成纤维细胞（HF）中糖胺聚糖（GAG）合成的因子。黑色素瘤MM - 96和结肠癌HT - 29的条件生长培养基分别使HF的GAG合成增加了10倍和8倍，对透明质酸的刺激作用更大。黑色素瘤FME以及乳腺癌MCF - 7和T - 47D的条件培养基使GAG合成增加了2倍，而结肠癌HCT - 8的培养基产生的反应不一，通常对GAG水平没有影响。HF培养物的条件培养基对肿瘤细胞的GAG合成没有影响。肿瘤细胞与HF共培养也导致GAG合成增加，刺激程度与条件培养基相似。肿瘤细胞条件培养基对培养中的猪平滑肌细胞和鸡胚成纤维细胞的GAG合成也有刺激作用，尽管GAG合成的增加幅度远小于HF培养物。这些发现支持了这样一种概念，即体内许多生长和侵袭性肿瘤所特有的基质纤维组织增生是由肿瘤细胞对周围正常结缔组织细胞的GAG合成进行调节而产生的。
