Deng Lishi, Taelman Steff, Olm Matthew R, Toe Laeticia Celine, Balini Eva, Ouédraogo Lionel Olivier, Bastos-Moreira Yuri, Argaw Alemayehu, Tesfamariam Kokeb, Sonnenburg Erica D, Hanley-Cook Giles T, Ouédraogo Moctar, Ganaba Rasmané, Van Criekinge Wim, Huybregts Lieven, Stock Michiel, Kolsteren Patrick, Sonnenburg Justin L, Lachat Carl, Dailey-Chwalibóg Trenton
Department of Food Technology, Safety and Health, Faculty of Bioscience Engineering, Ghent University, 9000, Ghent, Belgium.
BIOBIX, Department of Data Analysis and Mathematical Modelling, Ghent University, 9000, Ghent, Belgium.
Nat Commun. 2025 Mar 18;16(1):2683. doi: 10.1038/s41467-025-57838-y.
Balanced energy-protein (BEP) supplementation during pregnancy and lactation can improve birth outcomes and infant growth, with the gut microbiome as a potential mediator. The MISAME-III randomized controlled trial (ClinicalTrial.gov: NCT03533712) assessed the effect of BEP supplementation, provided during pregnancy and the first six months of lactation, on small-for-gestational age prevalence and length-for-age Z-scores at six months in rural Burkina Faso. Nested within MISAME-III, this sub-study examines the impact of BEP supplementation on maternal and infant gut microbiomes and their mediating role in birth outcomes and infant growth. A total of 152 mother-infant dyads (n = 71 intervention, n = 81 control) were included for metagenomic sequencing, with stool samples collected at the second and third trimesters, and at 1-2 and 5-6 months postpartum. BEP supplementation significantly altered maternal gut microbiome diversity, composition, and function, particularly those with immune-modulatory properties. Pathways linked to lipopolysaccharide biosynthesis were depleted and the species Bacteroides fragilis was enriched in BEP-supplemented mothers. Maternal BEP supplementation also accelerated infant microbiome changes and enhanced carbohydrate metabolism. Causal mediation analyses identified specific taxa mediating the effect of BEP on birth outcomes and infant growth. These findings suggest that maternal supplementation modulates gut microbiome composition and influences early-life development in resource-limited settings.
孕期和哺乳期补充能量-蛋白质平衡（BEP）可改善分娩结局和婴儿生长，肠道微生物群可能是其中介因素。MISAME-III随机对照试验（ClinicalTrial.gov：NCT03533712）评估了在布基纳法索农村地区，孕期和哺乳期前六个月补充BEP对小于胎龄儿患病率以及婴儿六个月龄时年龄别身长Z评分的影响。在MISAME-III试验中，这项子研究考察了补充BEP对母婴肠道微生物群的影响及其在分娩结局和婴儿生长中的中介作用。共有152对母婴二元组（n = 71干预组，n = 81对照组）纳入宏基因组测序，在孕中期和孕晚期以及产后1 - 2个月和5 - 6个月采集粪便样本。补充BEP显著改变了母亲肠道微生物群的多样性、组成和功能，尤其是那些具有免疫调节特性的微生物群。与脂多糖生物合成相关的途径减少，补充BEP的母亲体内脆弱拟杆菌富集。母亲补充BEP还加速了婴儿微生物群的变化并增强了碳水化合物代谢。因果中介分析确定了介导BEP对分娩结局和婴儿生长影响的特定分类群。这些发现表明，在资源有限的环境中，母亲补充营养可调节肠道微生物群组成并影响早期生命发育。
