Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
Gemini centre for Sepsis Research, Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway.
Commun Biol. 2022 Sep 8;5(1):932. doi: 10.1038/s42003-022-03865-0.
Complex carbohydrates that escape small intestinal digestion, are broken down in the large intestine by enzymes encoded by the gut microbiome. This is a symbiotic relationship between microbes and host, resulting in metabolic products that influence host health and are exploited by other microbes. However, the role of carbohydrate structure in directing microbiota community composition and the succession of carbohydrate-degrading microbes, is not fully understood. In this study we evaluate species-level compositional variation within a single microbiome in response to six structurally distinct carbohydrates in a controlled model gut using hybrid metagenome assemblies. We identified 509 high-quality metagenome-assembled genomes (MAGs) belonging to ten bacterial classes and 28 bacterial families. Bacterial species identified as carrying genes encoding starch binding modules increased in abundance in response to starches. The use of hybrid metagenomics has allowed identification of several uncultured species with the functional potential to degrade starch substrates for future study.
未能被小肠消化的复杂碳水化合物，在大肠中被肠道微生物组编码的酶所分解。这是微生物和宿主之间的共生关系，产生的代谢产物会影响宿主健康，并被其他微生物利用。然而，碳水化合物结构在指导微生物群落组成和碳水化合物降解微生物的演替方面的作用还不完全清楚。在这项研究中，我们使用混合宏基因组组装，在受控的模型肠道中评估了单一微生物组中 6 种结构不同的碳水化合物对物种水平组成变化的影响。我们鉴定了 509 个高质量的宏基因组组装基因组（MAG），属于 10 个细菌纲和 28 个细菌科。鉴定出携带淀粉结合模块基因的细菌物种，其丰度会因淀粉的存在而增加。混合宏基因组学的使用允许鉴定出几种具有潜在功能的未培养物种，这些物种可能具有降解淀粉底物的功能，可供未来研究使用。
