Guo Chunliang, Yong Wen, Yao Bihui, Song Lei, Liang Lu
Department of General Surgery, Baotou Central Hospital, No. 61 of Ring Road,Donghe District, Baotou, 014040, China.
Department of Neurology, The First Affiliated Hospital Of Baotou Medical College, Baotou, 014010, China.
BMC Gastroenterol. 2025 Mar 18;25(1):181. doi: 10.1186/s12876-025-03714-4.
This study aims to identify specific bile acids with potential early diagnostic value for acute pancreatitis (AP) and to provide a foundation for improved early diagnosis and the development of future therapeutic targets.
Targeted quantitative analysis of serum bile acids was performed using ultra-performance liquid chromatography coupled with high-resolution mass spectrometry in healthy individuals and individuals diagnosed with mild acute pancreatitis (MAP), moderate-to-severe acute pancreatitis (MSAP), and severe acute pancreatitis (SAP). Comparative analysis of bile acid profiles was conducted across these groups. The diagnostic performance of differential metabolic markers was evaluated using receiver operating characteristic curve analysis. Additionally, correlation heatmap analysis was employed to investigate associations between specific bile acids and clinical laboratory parameters.
Fourteen specific bile acids were identified. Taurocholic acid (TCA) was determined to be a distinguishing metabolite between the MSAP group and the healthy control group. Furthermore, taurochenodeoxycholic acid (TCDCA), glycocholic acid, taurodeoxycholic acid, and TCA were identified as differential metabolites between the SAP group and the healthy control group. Correlation analysis demonstrated that in the MSAP group, TCDCA exhibited a positive association with serum glucose, taurolithocholic acid (TLCA), serum triglycerides, cholic acid, and serum total cholesterol. In the SAP group, positive correlations were observed among TLCA, glycochenodeoxycholic acid, and serum calcium, between glycodeoxycholic acid (GDCA), chenodeoxycholic acid, and urine amylase, as well as between GDCA and serum lipase.
Specific serum bile acids, particularly TCA and TCDCA, demonstrate potential as biomarkers for the early, non-invasive, and accurate diagnosis of MSAP and SAP. These findings contribute to the advancement of early diagnostic strategies for acute pancreatitis.
本研究旨在识别对急性胰腺炎（AP）具有潜在早期诊断价值的特定胆汁酸，为改善早期诊断及未来治疗靶点的开发提供依据。
采用超高效液相色谱联用高分辨率质谱对健康个体以及诊断为轻度急性胰腺炎（MAP）、中重度急性胰腺炎（MSAP）和重度急性胰腺炎（SAP）的个体进行血清胆汁酸的靶向定量分析。对这些组别的胆汁酸谱进行比较分析。使用受试者工作特征曲线分析评估差异代谢标志物的诊断性能。此外，采用相关性热图分析来研究特定胆汁酸与临床实验室参数之间的关联。
鉴定出14种特定胆汁酸。牛磺胆酸（TCA）被确定为MSAP组与健康对照组之间的一种区分性代谢物。此外，牛磺鹅去氧胆酸（TCDCA）、甘氨胆酸、牛磺去氧胆酸和TCA被鉴定为SAP组与健康对照组之间的差异代谢物。相关性分析表明，在MSAP组中，TCDCA与血清葡萄糖、牛磺石胆酸（TLCA）、血清甘油三酯、胆酸和血清总胆固醇呈正相关。在SAP组中，TLCA、甘氨鹅去氧胆酸和血清钙之间，甘氨脱氧胆酸（GDCA）、鹅去氧胆酸和尿淀粉酶之间，以及GDCA和血清脂肪酶之间均观察到正相关。
特定血清胆汁酸，尤其是TCA和TCDCA，显示出作为MSAP和SAP早期、非侵入性及准确诊断生物标志物的潜力。这些发现有助于推进急性胰腺炎的早期诊断策略。
