Macdonald I A, Lake D M
J Neurosci Methods. 1985 May;13(3-4):239-48. doi: 10.1016/0165-0270(85)90072-x.
This paper describes a technique for selectively extracting catecholamines from body fluids prior to quantification by high-performance liquid chromatography with electrochemical detection. The technique is a two-stage process, the first stage involves the extraction of cations from the sample whilst the second stage is a liquid-liquid extraction involving the complexation of the cationic catecholamines with diphenylborate. This technique provides a very specific extraction procedure which results in chromatograms with no interfering compounds, and gives absolute recoveries of 70-80% for noradrenaline (NA), adrenaline (A) and dihydroxybenzylamine (internal standard), with similar relative recoveries of the 3 compounds. The intra-assay coefficients of variation for the measurement of catecholamines in venous plasma taken from resting subjects, are 8-9% for both NA and A, whilst the inter-assay values are 8% for NA and 20% for A.
本文描述了一种在通过高效液相色谱-电化学检测进行定量之前，从体液中选择性提取儿茶酚胺的技术。该技术分为两个阶段，第一阶段是从样品中提取阳离子，而第二阶段是液-液萃取，涉及阳离子儿茶酚胺与二苯基硼酸盐的络合。该技术提供了一种非常特异的提取方法，可得到无干扰化合物的色谱图，去甲肾上腺素（NA）、肾上腺素（A）和二羟基苄胺（内标）的绝对回收率为70-80%，这3种化合物的相对回收率相似。对于静息受试者采集的静脉血浆中儿茶酚胺的测量，NA和A的批内变异系数均为8-9%，而NA的批间值为8%，A的批间值为20%。
