Ahmed Nasar, Nazir Natasha, Asif Muhammad, Adnan M, Fakhar-E-Alam M, Zubair Muhammad, Ul Haq Khizar, Aseer Muhammad, Atif Muhammad, Ali Safdar
Department of Physics, King Abdullah Campus, The University of Azad Jammu and Kashmir, Muzaffarabad 13100, Pakistan.
Department of Physics, GC University Faisalabad, Faisalabad 38000, Pakistan.
Curr Pharm Des. 2025 Mar 17. doi: 10.2174/0113816128330548250206101727.
In this study, pure and cobalt manganese-doped ZnO nanoparticles (Zn(1-x-y)MnxCoyO NPs) at varying concentrations were synthesized through sol-gel method, and zinc acetate dihydrate, manganese nitrate, cobalt acetate, and diethyl amine were used as precursors, with samples finally calcined at 700oC.
The hexagonal wurtzite structure of pure and co-doped ZnO NPs was confirmed by X-ray diffraction (XRD). The computed grain sizes of pure and co-doped ZnO NPs, according to Scherrer's formula, were 32 nm, 32.5 nm, 36.3 nm, and 36.5 nm, respectively. SEM was used to observe the morphology of nanoparticles. FTIR spectroscopy was used to examine the chemical make-up and vibrational modes of pure and co-doped ZnO NPs. The bandgaps of pure and doped ZnO were examined using UV-Vis spectroscopy.
It was found that the optical bandgap of ZnO was lowered by 3.21 eV by manganese and cobalt doping. Elemental composition analysis was performed by using EDX analysis. Finally, anticancer activity of pure and co-doped ZnO NPs was assessed by employing MTT assay, which indicated that Zn0.8 Mn0.1 Co0.1O NPs showed significant anticancer results against liver cancer (HepG-2) cells as compared to ZnO, Zn0.98 Mn0.01Co0.01O and Zn0.90 Mn0.05 Co0.05O NPs. Moreover, Zn0.8 Mn0.1 Co0.1O NPs showed low toxicity and good biocompatibility comparable to doxorubicin (DOX).
Comprehensive experimental findings have demonstrated an authentic way of obtaining feasible in vivo liver cancer therapy.
在本研究中，通过溶胶-凝胶法合成了不同浓度的纯氧化锌纳米颗粒以及钴锰掺杂的氧化锌纳米颗粒（Zn(1-x-y)MnxCoyO NPs），使用二水合醋酸锌、硝酸锰、醋酸钴和二乙胺作为前驱体，样品最终在700℃下煅烧。
通过X射线衍射（XRD）确认了纯氧化锌纳米颗粒和共掺杂氧化锌纳米颗粒的六方纤锌矿结构。根据谢乐公式计算得出，纯氧化锌纳米颗粒和共掺杂氧化锌纳米颗粒的晶粒尺寸分别为32nm、32.5nm、36.3nm和36.5nm。使用扫描电子显微镜（SEM）观察纳米颗粒的形态。利用傅里叶变换红外光谱（FTIR）对纯氧化锌纳米颗粒和共掺杂氧化锌纳米颗粒的化学组成和振动模式进行检测。使用紫外-可见光谱（UV-Vis）检测纯氧化锌和掺杂氧化锌的带隙。
发现通过锰和钴掺杂，氧化锌的光学带隙降低了3.21eV。使用能谱分析（EDX）进行元素组成分析。最后，采用MTT法评估纯氧化锌纳米颗粒和共掺杂氧化锌纳米颗粒的抗癌活性，结果表明，与氧化锌、Zn0.98Mn0.01Co0.01O和Zn0.90Mn0.05Co0.05O纳米颗粒相比，Zn0.8Mn0.1Co0.1O纳米颗粒对肝癌（HepG-2）细胞显示出显著的抗癌效果。此外，Zn0.8Mn0.1Co0.1O纳米颗粒显示出与阿霉素（DOX）相当的低毒性和良好的生物相容性。
综合实验结果表明，找到了一种获得可行的体内肝癌治疗方法的可靠途径。
