Li Huan, Feng Yi, Li Qi, Wu Jianjun, Xing Hui, Liao Lingjie, Wang Zheng
National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Anhui Provincial Center for Disease Control and Prevention, Hefei City, Anhui Province, China.
China CDC Wkly. 2025 Feb 21;7(8):283-289. doi: 10.46234/ccdcw2025.046.
The genetic diversity of human immunodeficiency virus-1 (HIV-1) in China is characterized by multiple subtypes, circulating recombinant forms (CRFs) and unique recombinant forms (URFs) across the country. Through timely molecular surveillance, over 65 distinct CRFs have been identified in China to date. In this study, we identified five novel URFs among newly reported HIV-1 infected individuals in Anhui Province, China.
Near-full length HIV genome sequences were obtained using two-half molecule amplification methods from five samples containing potential URFs. The sequences were subsequently subjected to phylogenetic and recombination analyses.
Phylogenetic and recombination analyses of the five near-full length genome sequences confirmed their classification as novel URFs. Among these, three sequences were recombinants of CRF01_AE and CRF07_BC, one sequence was a recombinant of CRF01_AE, CRF07_BC and B, and one sequence resulted from CRF07_BC and CRF08_BC recombination.
The identification of URFs in newly infected individuals indicates ongoing transmission of multiple HIV-1 clades in Anhui Province, with superinfection occurring at notable frequencies. These findings emphasize the importance of enhancing long-term surveillance of circulating HIV-1 clades using near-full length sequence analysis in Anhui, China.
中国人类免疫缺陷病毒1型（HIV-1）的基因多样性表现为全国范围内存在多种亚型、流行重组型（CRF）和独特重组型（URF）。通过及时的分子监测，迄今为止在中国已鉴定出65种以上不同的CRF。在本研究中，我们在中国安徽省新报告的HIV-1感染者中鉴定出5种新型URF。
使用两半分子扩增方法从5个含有潜在URF的样本中获得近乎全长的HIV基因组序列。随后对这些序列进行系统发育和重组分析。
对这5个近乎全长基因组序列的系统发育和重组分析证实它们属于新型URF。其中，3个序列是CRF01_AE和CRF07_BC的重组体，1个序列是CRF01_AE、CRF07_BC和B的重组体，1个序列是CRF07_BC和CRF08_BC重组的结果。
在新感染个体中鉴定出URF表明安徽省多种HIV-1分支正在持续传播，超级感染发生频率显著。这些发现强调了在中国安徽省使用近乎全长序列分析加强对流行HIV-1分支进行长期监测的重要性。
