Boğa Kuru Buket, Makav Mustafa, Kuru Mushap, Aras Şükran Yediel, Karadağ Sarı Ebru, Bulut Menekşe, Alwazeer Duried, Bektaşoğlu Fikret, Ölmez Mükremin, Kırmızıbayrak Turgut, LeBaron Tyler W
Department of Animal Breeding and Husbandry, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye.
Department of Physiology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye.
Vet Med Sci. 2025 Mar;11(2):e70305. doi: 10.1002/vms3.70305.
This study investigated the impact of drinking hydrogen-rich water (HRW) on growth performance, organ weights, thiol/disulphide homeostasis, oxidative status and some hormonal, histopathological and immunohistochemical changes in rats fed in a restricted housing environment.
The eight groups (each group [male/female] eight rats) comprised two control, two hydrogen, two stress and two stress + hydrogen. All animals were given feed and water ad libitum for 3 months. Control and HRW group rats were calculated according to weight and housed according to the Guide's housing condition. The stress group and stress + HRW group were housed in half the area of the Guide's housing condition according to their weight. The animal's weekly body weights were measured throughout the study. The animals were sacrificed in accordance with ethical rules. Then, biochemical analyses were performed on thyroid-stimulating hormone (TSH), free triiodothyronine (FT3), free thyroxine (FT4), cortisol, parathyroid hormone (PTH) and calcium (Ca), total thiol (TT), native thiol (NT), disulphide, disulphide/TT × 100, disulphide/NT × 100 and NT/TT × 100, malondialdehyde (MDA) and glutathione (GSH). Haematoxylin staining for histopathological and SOD-2 immunoreactivity was also assessed.
Results showed that live weight gain was higher in the HRW groups than in the stress group. Oxidant status in biochemical analyses decreased in the stress + HRW group compared to the stress group. TSH decreased in the stress group. FT4, cortisol and Ca increased in the stress group.
The stress-related physiological parameters were reduced in the HRW + stress group compared to the stress group. HRW could be suggested when the organism is found in stressful conditions.
本研究调查了饮用富氢水（HRW）对处于受限饲养环境的大鼠生长性能、器官重量、硫醇/二硫键稳态、氧化状态以及一些激素、组织病理学和免疫组化变化的影响。
八组（每组[雄性/雌性]八只大鼠）包括两个对照组、两个富氢组、两个应激组和两个应激+富氢组。所有动物自由采食和饮水3个月。根据体重计算对照组和HRW组大鼠，并按照指南的饲养条件进行饲养。应激组和应激+HRW组根据体重饲养在指南规定饲养面积的一半空间内。在整个研究过程中每周测量动物体重。按照伦理规则处死动物。然后，对促甲状腺激素（TSH）、游离三碘甲状腺原氨酸（FT3）、游离甲状腺素（FT4）、皮质醇、甲状旁腺激素（PTH）和钙（Ca）、总硫醇（TT）、天然硫醇（NT）、二硫键、二硫键/TT×100、二硫键/NT×100和NT/TT×100、丙二醛（MDA）和谷胱甘肽（GSH）进行生化分析。还评估了苏木精染色的组织病理学和超氧化物歧化酶2（SOD-2）免疫反应性。
结果显示，HRW组的体重增加高于应激组。与应激组相比，应激+HRW组生化分析中的氧化状态降低。应激组的TSH降低。应激组的FT4、皮质醇和Ca升高。
与应激组相比，HRW+应激组与应激相关的生理参数降低。当机体处于应激状态时，建议饮用HRW。
