Chen Jie, Wu Wenjing, Peng Yongzhen, Fang Zheng, Hu Yujing, Guo Kai
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, Jiangsu, 211816, P. R. China.
State Key Laboratory of Materials-Oriented Chemical Engineering Institution, Nanjing Tech University, Nanjing, Jiangsu, 210009, P. R. China.
Chemistry. 2025 May;31(25):e202500539. doi: 10.1002/chem.202500539. Epub 2025 Mar 30.
Flavin-dependent ene-reductases (EREDs), members of the Old Yellow Enzyme (OYE) superfamily, are highly efficient biocatalysts primarily known for catalyzing the asymmetric reduction of activated alkenes. Beyond this native function, the chemical versatility of the flavin cofactor and the sophisticated architecture of their protein structures enable EREDs to exhibit catalytic multifunctionality. The catalytic promiscuity not only highlights the adaptability of these enzymes but also expands their potential to broaden the scope of enzyme-catalyzed reactions in organic synthesis. Given the inherent challenges associated with discovering novel enzyme activities, such catalytic promiscuity of EREDs offers a promising pathway for expanding their applications. This mini-review provides a comprehensive overview of the catalytic multifunctionality of flavin-dependent EREDs, with a particular focus on their "non-natural" functionalities in organic synthesis. This review is primarily divided into two main sections: hydride-dependent reactions and hydride-independent reactions. By highlighting these unconventional biocatalytic pathways, we aim to inspire further exploration into the untapped potential of EREDs and their role in advancing synthetic chemistry.
黄素依赖性烯还原酶（EREDs）是老黄色酶（OYE）超家族的成员，是高效的生物催化剂，主要以催化活化烯烃的不对称还原而闻名。除了这种天然功能外，黄素辅因子的化学多功能性及其蛋白质结构的复杂架构使EREDs能够展现出催化多功能性。催化多效性不仅突出了这些酶的适应性，还拓展了它们在有机合成中扩大酶催化反应范围的潜力。鉴于发现新酶活性存在内在挑战，EREDs的这种催化多效性为扩展其应用提供了一条有前景的途径。本综述简要概述了黄素依赖性EREDs的催化多功能性，特别关注它们在有机合成中的“非天然”功能。本综述主要分为两个主要部分：氢化物依赖性反应和氢化物非依赖性反应。通过强调这些非常规的生物催化途径，我们旨在激发对EREDs未开发潜力及其在推进合成化学中作用的进一步探索。
