Lauková Andrea, Focková Valentína, Maďar Marián, Belzecki Grzegorz, Miltko Renata, Simonová Monika Pogány
Centre of Biosciences of the Slovak Academy of Sciences, Institute of Animal Physiology, Šoltésovej 4-6, 040 01, Košice, Slovakia.
Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81, Košice, Slovakia.
Vet Res Commun. 2025 Mar 19;49(3):141. doi: 10.1007/s11259-025-10701-3.
Roe and red deers represent inhabitants in European forests but also in agricultural areas. In general, microbiota can have a significant impact on health. E. g. the genus Enterococcus was detected in more or less high abundance percentage in feces of red deers. To preserve negative impact of some microbiota, beneficial autochthonous strains can be selected for this purpose. The aim of this study was to assess safety, postbiotic activity and character of the fecal strains Enterococcus mundtii from roe and red deers living in Poland to spread basic microbiology research in this field and to select application candidate. Taxonomy of seven E. mundtii was determined using MALDI-TOF mass spectrometry and 16S rRNA sequence analysis. The evaluation score responded mostly with secure genus identification/probable species identification. Strains showed identity up to 100% with the sequence MK414812.1 of E. mundtii in GenBank. They were deoxyribonuclease and gelatinase- negative, with low-grade biofilm formation (0.100 ± 0.31 to 0.181 ± 0.43). E. mundtii were susceptible to antibiotics without production of damaging enzymes. They were absent of virulence factors genes (gelE, agg, esp, efaAfm, efaAfs). Postbiotic potential of the bacteriocin substance produced by E. mundtii revealed inhibition of indicator strains growth up to 48%. The most active substance was from the strain EM 1/90/2; inhibitory activity against enterococci, staphylococci and listeriae up to 86% (up to 1600 AU mL) was noted. The substance from the strain EM 6/123/1 reached inhibitory activity up to 81% with activity up to 400 AU/mL. It remained active at -20 ˚C for one month. E. mundtii were assessed with no and/or low- grade virulence factor rate and with postbiotic potential for further studies.
狍和马鹿不仅是欧洲森林的居民，也是农业地区的居民。一般来说，微生物群会对健康产生重大影响。例如，在马鹿粪便中或多或少能检测到较高丰度百分比的肠球菌属。为了消除某些微生物群的负面影响，可以为此选择有益的本土菌株。本研究的目的是评估来自生活在波兰的狍和马鹿的蒙氏肠球菌粪便菌株的安全性、后生元活性和特性，以扩展该领域的基础微生物学研究并选择应用候选菌株。使用基质辅助激光解吸电离飞行时间质谱和16S rRNA序列分析确定了7株蒙氏肠球菌的分类学。评估分数大多对应可靠的属鉴定/可能的种鉴定。这些菌株与GenBank中蒙氏肠球菌的序列MK414812.1的同一性高达100%。它们脱氧核糖核酸酶和明胶酶呈阴性，生物膜形成程度低（0.100±0.31至0.181±0.43）。蒙氏肠球菌对抗生素敏感，不产生破坏性酶。它们没有毒力因子基因（gelE、agg、esp、efaAfm、efaAfs）。蒙氏肠球菌产生的细菌素物质的后生元潜力显示，对指示菌株生长的抑制率高达48%。活性最强的物质来自菌株EM 1/90/2；对肠球菌、葡萄球菌和李斯特菌的抑制活性高达86%（高达1600 AU/mL）。来自菌株EM 6/123/1的物质的抑制活性高达81%，活性高达400 AU/mL。它在-20℃下可保持活性一个月。蒙氏肠球菌被评估为无毒力因子或毒力因子率低且具有后生元潜力，可用于进一步研究。
