Wang Jinhong, Ma Zhihui, Xu Qianyue, Wei Benliang, Wang Mengmeng, Liu Yanhong, Tian Yu, Zhang Haifeng, Xiao Liang, Zhong Yiyi, Zou Yuanqiang
BGI Research, Shenzhen, 518083, China.
College of Life Sciences, Southwest University, Chongqing, 400715, China.
Probiotics Antimicrob Proteins. 2025 Mar 19. doi: 10.1007/s12602-025-10515-4.
Lacticaseibacillus rhamnosus is extensively studied, with some strains widely applied for enhancing human health. Complete genome analysis is crucial for the functional exploration of probiotics. This study aimed to investigate the potential of L. rhamnosus OF44 (OF44) to promote human health through complete genome and phenotypic analysis. The complete genome sequence of OF44 was 2,978,769 bp, with 2791 CDSs and an average GC content of 47%. In vitro experiments demonstrated that OF44 had a broad carbon source fermentation capacity. Resistance gene analysis and simulated digestion tests confirmed the favorable tolerance of OF44 under harsh conditions, suggesting its potential as an oral supplement. OF44 possessed various potential genes related to bioactive substances with antimicrobial activity, such as antimicrobial peptides, lactic acid, hydrogen peroxide, and exopolysaccharides, most of which were detected in vitro. Further, OF44 exhibited significant growth inhibition capacities against pathogens from the gut, vagina, skin, and mouth, likely due to high co-aggregation with pathogens, multiple antimicrobial peptide clusters, and adhesin gene clusters. Additionally, oral administration of OF44 was found to reduce the pH and inflammation levels in the vaginal microenvironment of rats with bacterial vaginosis. Therefore, OF44 exhibited probiotic properties in improving reproductive tract bacterial infections by modulating vaginal microbiota balance and inhibiting pathogen growth. In summary, this study provided a new perspective on the application of OF44 as a supplement in the food and pharmaceutical fields.
鼠李糖乳杆菌受到广泛研究，一些菌株被广泛应用于促进人类健康。完整的基因组分析对于益生菌的功能探索至关重要。本研究旨在通过完整的基因组和表型分析，探究鼠李糖乳杆菌OF44（OF44）促进人类健康的潜力。OF44的完整基因组序列为2,978,769 bp，有2791个编码序列，平均GC含量为47%。体外实验表明，OF44具有广泛的碳源发酵能力。抗性基因分析和模拟消化试验证实了OF44在恶劣条件下具有良好的耐受性，表明其作为口服补充剂的潜力。OF44拥有多种与具有抗菌活性的生物活性物质相关的潜在基因，如抗菌肽、乳酸、过氧化氢和胞外多糖，其中大部分在体外被检测到。此外，OF44对来自肠道、阴道、皮肤和口腔的病原体表现出显著的生长抑制能力，这可能是由于与病原体的高共聚集、多个抗菌肽簇和黏附素基因簇。另外，发现口服OF44可降低患有细菌性阴道炎的大鼠阴道微环境的pH值和炎症水平。因此，OF44通过调节阴道微生物群平衡和抑制病原体生长，在改善生殖道细菌感染方面表现出益生菌特性。总之，本研究为OF44作为食品和制药领域的补充剂的应用提供了新的视角。
