Chu Chunuo, Huang Shang, Wang Xin, Zhao Guoqiang, Hao Wenqi, Zhong Yiyi, Ma Zhihui, Huang Congfu, Peng Yuanping, Wei Fengxiang
International Department, Shenzhen Middle School, Shenzhen, China.
Department of Centre Lab, Longgang District Maternity & Child Healthcare Hospital of Shenzhen City, Shenzhen, China.
Transl Pediatr. 2024 Jan 29;13(1):72-90. doi: 10.21037/tp-23-566. Epub 2024 Jan 24.
Cerebral palsy (CP) is a unique neurological disorder which adversely affects motion. Cytokines and gut microbial composition contribute to CP and other diseases, such as reproductive tract inflammation and bone loss. Importantly, () reduces the degree of inflammation and improves overall health status. As our previous study showed that () OF44, a selected strain of gut bacteria originally used to treat reproductive tract inflammation and bone loss, has effects similar to that of , we decided to use OF44 on CP rats. Validation of the effects of OF44 on CP adds to its confirmed effects in treating osteoporosis and reproductive tract microbiota disorders, increasing its potential as a probiotic. The purpose of this was to ascertain whether OF44 can alleviate the symptoms of CP.
CP rat models were created through left carotid artery ligation. Following this, 100-day old CP rats were exposed to OF44, , or normal saline gastric gavage daily for 28 days. Grouping of the rats is determined randomly. Before and after the gavage, behavioral experiments were conducted and the inflammation levels assessed via measurements of interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor alpha (TNF-α) inflammatory markers. The efficacy of the outcome is measured by performing statistical analysis like the -test on the data to see its significance. Additionally, variations inside gut microbiome were evaluated via 16S ribosomal RNA sequencing.
Before intervention, CP rats failed to exhibit depression-like behavior (P=0.6). OF44 treatment significantly reduced the level of IL-6 (P=4.8e-05), treatment significantly reduced the level of TNF-α (P=0.04). In addition, both treatments altered the composition and complexity of the gut microbiome.
Our results indicated that OF44 has potential in alleviating inflammation and altering the gut microbial composition in CP, and that it has the potential to clinically treat CP. There are some limitations of this study. For example, dietary differences and their effects on gastrointestinal dysfunction are not considered in this study, and only two behavioral experiments were used.
脑瘫（CP）是一种对运动产生不利影响的独特神经障碍。细胞因子和肠道微生物组成与脑瘫及其他疾病有关，如生殖道炎症和骨质流失。重要的是，（）可减轻炎症程度并改善整体健康状况。正如我们之前的研究表明，（）OF44是一种最初用于治疗生殖道炎症和骨质流失的肠道细菌精选菌株，其效果与（）类似，我们决定将OF44用于脑瘫大鼠。验证OF44对脑瘫的作用，增加了其在治疗骨质疏松症和生殖道微生物群紊乱方面已得到证实的效果，提高了其作为益生菌的潜力。本研究的目的是确定OF44是否能缓解脑瘫症状。
通过左颈动脉结扎建立脑瘫大鼠模型。此后，对100日龄的脑瘫大鼠每天进行OF44、（）或生理盐水灌胃，持续28天。大鼠随机分组。在灌胃前后进行行为实验，并通过测量白细胞介素（IL）-1β、IL-6、IL-8和肿瘤坏死因子α（TNF-α）炎症标志物来评估炎症水平。通过对数据进行如t检验等统计分析来衡量结果的有效性，以查看其显著性。此外，通过16S核糖体RNA测序评估肠道微生物组内的变化。
干预前，脑瘫大鼠未表现出抑郁样行为（P = 0.6）。OF44治疗显著降低了IL-6水平（P = 4.8e - 05），（）治疗显著降低了TNF-α水平（P = 0.04）。此外，两种治疗均改变了肠道微生物组的组成和复杂性。
我们的结果表明，OF44在减轻脑瘫炎症和改变肠道微生物组成方面具有潜力，并且具有临床治疗脑瘫的潜力。本研究存在一些局限性。例如，本研究未考虑饮食差异及其对胃肠功能障碍的影响，且仅使用了两项行为实验。
