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IBEX知识库:多重成像技术的核心资源。

The IBEX Knowledge-Base: A central resource for multiplexed imaging techniques.

作者信息

Radtke Andrea J, Anidi Ifeanyichukwu U, Arakkal Leanne, Arroyo-Mejias Armando J, Beuschel Rebecca T, Börner Katy, Chu Colin J, Clark Beatrice, Clatworthy Menna R, Colautti Jake, Coscia Fabian, Croteau Joshua, Denha Saven, Dever Rose, Dutra Walderez O, Fritzsche Sonja, Fullam Spencer, Gerner Michael Y, Gola Anita, Gollob Kenneth J, Hernandez Jonathan M, Hor Jyh Liang, Ichise Hiroshi, Jing Zhixin, Jonigk Danny, Kandov Evelyn, Kastenmüller Wolfgang, Koenig Joshua F E, Kortekaas Rosa K, Kothurkar Aanandita, Kreins Alexandra Y, Lamborn Ian T, Lin Yuri, Luciano Pereira Morais Katia, Lunich Aleksandra, Luz Jean C S, MacDonald Ryan B, Makranz Chen, Maltez Vivien I, McDonough John E, Moriarty Ryan V, Ocampo-Godinez Juan M, Olyntho Vitoria M, Oxenius Annette, Padhan Kartika, Remmert Kirsten, Richoz Nathan, Schrom Edward C, Shang Wanjing, Shi Lihong, Shih Rochelle M, Speranza Emily, Stierli Salome, Teichmann Sarah A, Veres Tibor Z, Vierhout Megan, Wachter Brianna T, Wade-Vallance Adam K, Williams Margaret, Zangger Nathan, Germain Ronald N, Yaniv Ziv

机构信息

Lymphocyte Biology Section and Center for Advanced Tissue Imaging, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America.

Critical Care Medicine and Pulmonary Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America.

出版信息

PLoS Biol. 2025 Mar 19;23(3):e3003070. doi: 10.1371/journal.pbio.3003070. eCollection 2025 Mar.

DOI:10.1371/journal.pbio.3003070
PMID:40106489
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12135934/
Abstract

Multiplexed imaging is a powerful approach in spatial biology, although it is complex, expensive and labor-intensive. Here, we present the IBEX Knowledge-Base, a central resource for reagents, protocols and more, to enhance knowledge sharing, optimization and innovation of spatial proteomics techniques.

摘要

多重成像技术是空间生物学领域一种强大的方法,尽管它复杂、昂贵且耗费人力。在此,我们展示了IBEX知识库,这是一个关于试剂、实验方案等的核心资源库,旨在加强空间蛋白质组学技术的知识共享、优化和创新。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a00b/12135934/45b6ea022e5c/pbio.3003070.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a00b/12135934/45b6ea022e5c/pbio.3003070.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a00b/12135934/45b6ea022e5c/pbio.3003070.g001.jpg

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Method of the Year 2024: spatial proteomics.2024年度方法:空间蛋白质组学。
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A spatial human thymus cell atlas mapped to a continuous tissue axis.空间人类胸腺细胞图谱绘制到连续的组织轴上。
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'Iterative Bleaching Extends Multiplexity' facilitates simultaneous identification of all major retinal cell types.“迭代漂白扩展多重性”有助于同时识别所有主要视网膜细胞类型。
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Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging.器官绘图抗体面板:标准化多重组织成像的社区资源。
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IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues.IBEX:一种用于高内涵成像的迭代免疫标记和化学漂白方法,适用于多种组织。
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