IBEX成像知识库：一个促进免疫荧光成像方法应用与发展的社区资源。
The IBEX Imaging Knowledge-Base: A Community Resource Enabling Adoption and Development of Immunofluoresence Imaging Methods.
作者信息
Yaniv Ziv, Anidi Ifeanyichukwu U, Arakkal Leanne, Arroyo-Mejías Armando J, Beuschel Rebecca T, Börner Katy, Chu Colin J, Clark Beatrice, Clatworthy Menna R, Colautti Jake, Coscia Fabian, Croteau Joshua, Denha Saven, Dever Rose, Dutra Walderez O, Fritzsche Sonja, Fullam Spencer, Gerner Michael Y, Gola Anita, Gollob Kenneth J, Hernandez Jonathan M, Hor Jyh Liang, Ichise Hiroshi, Jing Zhixin, Jonigk Danny, Kandov Evelyn, Kastenmüller Wolfgang, Koenig Joshua F E, Kothurkar Aanandita, Kortekaas Rosa K, Kreins Alexandra Y, Lamborn Ian T, Lin Yuri, Morais Katia Luciano Pereira, Lunich Aleksandra, Luz Jean C S, MacDonald Ryan B, Makranz Chen, Maltez Vivien I, McDonough John E, Moriarty Ryan V, Ocampo-Godinez Juan M, Olyntho Vitoria M, Oxenius Annette, Padhan Kartika, Remmert Kirsten, Richoz Nathan, Schrom Edward C, Shang Wanjing, Shi Lihong, Shih Rochelle M, Speranza Emily, Stierli Salome, Teichmann Sarah A, Veres Tibor Z, Vierhout Megan, Wachter Brianna T, Williams Margaret, Zangger Nathan, Germain Ronald N, Radtke Andrea J
机构信息
Bioinformatics and Computational Bioscience Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Critical Care Medicine and Pulmonary Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
出版信息
ArXiv. 2024 Dec 17:arXiv:2412.12965v1.
The iterative bleaching extends multiplexity (IBEX) Knowledge-Base is a central portal for researchers adopting IBEX and related 2D and 3D immunofluorescence imaging methods. The design of the Knowledge-Base is modeled after efforts in the open-source software community and includes three facets: a development platform (GitHub), static website, and service for data archiving. The Knowledge-Base facilitates the practice of open science throughout the research life cycle by providing validation data for recommended and non-recommended reagents, e.g., primary and secondary antibodies. In addition to reporting negative data, the Knowledge-Base empowers method adoption and evolution by providing a venue for sharing protocols, videos, datasets, software, and publications. A dedicated discussion forum fosters a sense of community among researchers while addressing questions not covered in published manuscripts. Together, scientists from around the world are advancing scientific discovery at a faster pace, reducing wasted time and effort, and instilling greater confidence in the resulting data.
迭代漂白扩展多重性（IBEX）知识库是采用IBEX及相关二维和三维免疫荧光成像方法的研究人员的核心门户。该知识库的设计借鉴了开源软件社区的成果，包括三个方面：一个开发平台（GitHub）、静态网站和数据存档服务。该知识库通过为推荐和非推荐试剂（如一级和二级抗体）提供验证数据，在整个研究生命周期中促进开放科学的实践。除了报告负面数据外，该知识库还通过提供一个分享方案、视频、数据集、软件和出版物的场所，促进方法的采用和发展。一个专门的讨论论坛在研究人员中营造了一种社区感，同时解决已发表手稿中未涵盖的问题。来自世界各地的科学家们共同以更快的速度推进科学发现，减少了浪费的时间和精力，并增强了对所得数据的信心。
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