Department of Intelligent Systems Engineering, Indiana University, Bloomington, IN, USA.
Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.
Nat Methods. 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7. Epub 2023 Jul 19.
Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.
基于抗体的多重分析成像技术可以实现对组织中分子和细胞结构的详细特征分析。该领域的研究进展使得人们现在能够进行高参数数据采集（>60 个靶点）；然而，这些方法中使用的抗体面板需要相当的专业知识和资金来构建。器官绘图抗体面板是经过社区验证的资源，可以节省时间和金钱，提高重现性，加速发现，并支持构建人类参考图谱。
