Xiapyrroles A-F: -Alkylpyrrole Alkaloids from the Marine-Derived Actinomycete 1310KO-148.

Six new -alkylpyrrole alkaloids (-) were isolated from the marine-derived actinomycete 1310KO-148 from a sponge sample. The structures of xiapyrroles A-F (-) were elucidated by detailed analysis of extensive spectroscopic data, including 1D, 2D NMR, and HRESIMS data. The absolute configurations of , , , and were determined by a comparison of their calculated and experimental electronic circular dichroism (ECD) spectra. The position of the hydroxamate group in was confirmed through -methylation and NOESY data analysis. All compounds (-) were tested for their anti-inflammatory effects in LPS-stimulated RAW 264.7 cells, a mouse macrophage cell line. The treatment of RAW 264.7 cells with 30 μM of - showed no significant cytotoxic effects. However, dose-dependently suppressed the LPS-induced production of NO (IC = 29.5 μM) and interleukin-6 (IL-6) (IC = 10.9 μM). Compound exhibited no potential cytotoxicity against six solid cancer cell lines and eight blood cancer cell lines at a concentration of 30 μM.