Amomaxyz A and B, Sesquiterpene-Indole Alkaloids from Fruits of Roxb.

Two unprecedented 4/9/5-membered ring sesquiterpene-indole alkaloids, amomaxyz A () and B (), along with one known compound caryolane-1,9β-diol (), were isolated from the fruit of Roxb. Their structures and absolute configurations were elucidated through comprehensive spectroscopic analyses, including NMR, electronic circular dichroism (ECD), X-ray single-crystal diffraction, and DP4 probability analysis of calculated NMR data. Biological evaluation revealed distinct anti-inflammatory and anticancer activities. demonstrated dose-dependent anti-inflammatory activity by suppressing NO release in LPS/IFN-γ-activated RAW264.7 macrophages (25.9% at 10 μM, 29.9% at 20 μM; < 0.01), while and exhibited weaker effects. Moreover, (IC = 30.62 μM) and (IC = 23.43 μM) showed moderate inhibitory effects against HepG2 cells, which reveals the potential of their biological activity.