Erdemir Serkan, Oguz Mehmet, Malkondu Sait
Selcuk University, Science Faculty, Department of Chemistry, 42250, Konya, Turkey.
Selcuk University, Science Faculty, Department of Chemistry, 42250, Konya, Turkey.
Talanta. 2025 Sep 1;292:127906. doi: 10.1016/j.talanta.2025.127906. Epub 2025 Mar 12.
Biogenic amines (BAs) are critical biomolecules that play key roles in physiological processes and serve as important indicators in food safety, clinical diagnostics, and environmental monitoring. Therefore, sensitive, selective, and rapid tools are required for BA detection. This study explores the synthesis and applications of a new fluorescent probe (DBQ) for detecting BAs, focusing on their fluorescence response mechanisms. DBQ offers a promising alternative due to its high selectivity, sharp color change, low detection limit (0.057 μM for cadaverine), long lifetime (τ ≈ 2.40 ns), rapid response (15 min), low cytotoxicity (over 90 % cell viability in the presence of 10.0 μM of DBQ) and favorable photophysical properties, including large Stokes shift (213 nm in CHCl). In addition, DBQ displays solvent-dependent intramolecular charge transfer (ICT), resulting in solvatochromism. The developed smartphone sensing system was applied to the detection of BAs. The developed sensing test kit responds quickly to the presence of volatile biogenic amines, with notable visible response and high selectivity. In on-site analysis, we were able to successfully use these test strips for non-destructive evaluation of chicken and cheese freshness with the use of a smartphone. Therefore, the current study will contribute to improving food safety and reduce food loss and waste by developing new bioanalytical technologies that offer chemical information about the composition of food, which is extremely valuable for enhancing traceability and extending food shelf life.
生物胺（BAs）是关键的生物分子，在生理过程中发挥着关键作用，同时也是食品安全、临床诊断和环境监测中的重要指标。因此，需要灵敏、选择性好且快速的工具来检测生物胺。本研究探索了一种用于检测生物胺的新型荧光探针（DBQ）的合成与应用，重点关注其荧光响应机制。DBQ因其高选择性、明显的颜色变化、低检测限（尸胺的检测限为0.057 μM）、长寿命（τ≈2.40 ns）、快速响应（15分钟）、低细胞毒性（在10.0 μM DBQ存在下细胞活力超过90%）以及良好的光物理性质，包括大斯托克斯位移（在CHCl中为213 nm），提供了一种有前景的替代方法。此外，DBQ表现出溶剂依赖性分子内电荷转移（ICT），导致溶剂致变色。所开发的智能手机传感系统被应用于生物胺的检测。所开发的传感测试试剂盒对挥发性生物胺的存在响应迅速，具有明显的可见响应和高选择性。在现场分析中，我们能够使用智能手机成功地将这些测试条用于鸡肉和奶酪新鲜度的无损评估。因此，当前的研究将通过开发新的生物分析技术来改善食品安全并减少食物损失和浪费，这些技术能够提供有关食物成分的化学信息，这对于提高可追溯性和延长食物保质期极为有价值。
