Sun Shan, Yang Dan, Li Zheng, Cheng Wei, Yang Yanli, Li Ling, Luo Xiangjie, Qian Yong
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210046 China.
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210046 China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Apr 15;331:125808. doi: 10.1016/j.saa.2025.125808. Epub 2025 Jan 27.
Biogenic amines (BAs) are a class of nitrogen-containing natural organic compounds. Elevated levels of BAs are a reliable indicator of food spoilage and pose a significant risk to human health. Thus, the development of real-time sensors for BAs monitoring is crucial. In this study, we present a novel fluorescence probe, TP-A, based on ylidenemalononitrile enamine, which can detect various BAs including putrescine, cadaverine, and spermine. TP-A exhibits a significant fluorescence intensity increase at 600 nm in the presence of these BAs in an aqueous system, which can effectively monitor BAs in representative vegetable onion tissues. Notably, TP-A-loaded filter paper has been developed into test strips for real-time monitoring of BAs released from shrimp under different temperatures. This work offers a precise method for probing the release of BAs in real food samples, which is promising for application in food quality control.
生物胺（BAs）是一类含氮的天然有机化合物。生物胺水平升高是食品变质的可靠指标，对人类健康构成重大风险。因此，开发用于生物胺监测的实时传感器至关重要。在本研究中，我们提出了一种基于亚甲基丙二腈烯胺的新型荧光探针TP-A，它可以检测包括腐胺、尸胺和精胺在内的各种生物胺。在水体系中，当存在这些生物胺时，TP-A在600nm处的荧光强度显著增加，这可以有效地监测代表性蔬菜洋葱组织中的生物胺。值得注意的是，负载TP-A的滤纸已被开发成测试条，用于实时监测不同温度下虾释放的生物胺。这项工作为探测实际食品样品中生物胺的释放提供了一种精确方法，有望应用于食品质量控制。
