Akköse Mehmet
Department of Livestock, Dalaman Agricultural Enterprise, General Directorate of Agricultural Enterprises, 48770 Dalaman, Muğla, Türkiye.
J Equine Vet Sci. 2025 Jun;149:105417. doi: 10.1016/j.jevs.2025.105417. Epub 2025 Mar 17.
Foals, that receive poor-quality colostrum, are exposed to a risk of failure of transfer of passive immunity. The Brix refractometer can be used for the estimation of the immunoglobulin (IgG) concentration of mare colostrum, yet there is a scarce literature available on Brix refractometer thresholds for use in the detection of mare colostra with high and low IgG concentrations.
This study aimed to assess the diagnostic accuracy of the Brix refractometer in determining mare colostra with IgG concentrations of <50g/L, <60g/L, ≥80g/L, ≥100g/L and ≥125g/L.
Two hundred and seventeen Arabian mares had colostral IgG concentrations measured with the radial immunodiffusion assay and estimated with the Brix refractometer. Brix thresholds were determined by performing a receiver operating characteristics analysis.
The mean colostral IgG concentration was 83.73 ± 2.46 g/L and colostral IgG concentrations had a high correlation (Pearson's r=0.78) with Brix percentages. The optimal refractometer thresholds for the determination of colostral IgG concentrations of <50, <60, ≥80, ≥100 and ≥125 g/L were ascertained to be ≤19.6 %, ≤20.1 %, >21.6 %, >23.6 % and >27.3 %, respectively. The sensitivity and specificity of the Brix refractometer for the determination of colostral IgG concentrations of <50, <60, ≥80, ≥100 and ≥125 g/L were found to be 89.3 % (95 % CI: 71.8-97.7 %) and 83.8 % (95 % CI: 77.5-89.0 %); 82.0 % (95 % CI: 68.6-91.4 %) and 88.1 % (95 % CI: 81.8-92.8 %); 85.3 % (95 % CI: 76.9-91.5 %) and 73.7 % (95 % CI: 63.9-82.1 %); 84.8 % (95 % CI: 73.0-92.8 %) and 76.1 % (95 % CI: 68.2-82.8 %); and 75.0 % (95 % CI: 53.3-90.2 %) and 90.4 % (95 % CI: 85.1-94.3 %), respectively.
The digital Brix refractometer showed a good performance in determining mare colostra with low and high IgG concentrations.
摄入低质量初乳的马驹面临被动免疫转移失败的风险。白利糖度折射仪可用于估算母马初乳中的免疫球蛋白（IgG）浓度，但关于用于检测IgG浓度高低的母马初乳白利糖度折射仪阈值的文献却很少。
本研究旨在评估白利糖度折射仪在测定IgG浓度<50g/L、<60g/L、≥80g/L、≥100g/L和≥125g/L的母马初乳时的诊断准确性。
对217匹阿拉伯母马的初乳IgG浓度进行放射免疫扩散测定，并使用白利糖度折射仪进行估算。通过进行受试者工作特征分析来确定白利糖度阈值。
初乳IgG平均浓度为83.73±2.46g/L，初乳IgG浓度与白利糖度百分比具有高度相关性（皮尔逊r=0.78）。确定用于测定IgG浓度<50、<60、≥80、≥100和≥125g/L的初乳的最佳折射仪阈值分别为≤19.6%、≤20.1%、>21.6%、>23.6%和>27.3%。发现白利糖度折射仪用于测定IgG浓度<50、<60、≥80、≥100和≥125g/L的初乳时的灵敏度和特异性分别为89.3%（95%CI：71.8-97.7%）和83.8%（95%CI：77.5-89.0%）；82.0%（95%CI：68.6-91.4%）和88.1%（95%CI：81.8-92.8%）；85.3%（95%CI：76.9-91.5%）和73.7%（95%CI：63.9-82.1%）；84.8%（95%CI：73.0-92.8%）和76.1%（95%CI：68.2-82.8%）；以及75.0%（95%CI：53.3-90.2%）和90.4%（95%CI：85.1-94.3%）。
数字白利糖度折射仪在测定IgG浓度高低的母马初乳方面表现良好。
