Miyaji M, Nishimura K
Mycopathologia. 1985 May;90(2):121-3. doi: 10.1007/BF00436863.
The morphological conversion of Coccidioides immitis in vivo was observed using the agar-implantation method. Short hyphae implanted into the peritoneal cavity of mice swelled gradually and many septa appeared in them after 48 hours. As the implantation period advanced, these arthroconidia swelled and then broke up into spherical cells at the 72nd hour. On the other hand, arthroconidia implanted into the peritoneal cavity of mice also swelled and became immature spherules within 72 hours. During the 96th hour a few of the spherules reached a stage of maturity. They were filled with numerous endospores. The agar-implantation method is therefore a useful for the demonstration of spherules of C. immitis.
采用琼脂植入法观察了荚膜组织胞浆菌在体内的形态转化。植入小鼠腹腔的短菌丝逐渐肿胀，48小时后出现许多隔膜。随着植入时间的延长，这些关节孢子在72小时时肿胀，然后分裂成球形细胞。另一方面，植入小鼠腹腔的关节孢子也会肿胀，并在72小时内变成未成熟的球形体。在第96小时，一些球形体达到成熟阶段。它们充满了大量的内生孢子。因此，琼脂植入法对于显示荚膜组织胞浆菌的球形体很有用。
