Miyaji M, Nishimura K
Mycopathologia. 1977 Feb 18;60(2):73-8. doi: 10.1007/BF00490375.
By the agar-implantation developed by the authors the process of conversion on Blastomyces dermatitidis from mycelial phase to yeast phase was observed. First of all slide cultures of the fungus were prepared at room temperature. Upon confirmation of good hyphal growth, a cover glass was removed and a part of medium was cut out in a square of about 3 mm a side. After mice were laparotomied, each agar block cut out was implanted in the peritoneal cavity of mouse. The mice implanted with the agar blocks were killed, two each, every day for 14 days, and thereafter at intervals of a week for 2 months. Therefore, the implanted agar blocks were all recovered. They were examined directly by a light microscope with histopathological and electron microscopic examinations carried out at the same time. Within the peritoneal cavity of mouse, the intercalary and terminal chlamydospores were formed from hyphae. These subsequently swelled to become yeastlike cells and proliferated thereafter by budding.
通过作者开发的琼脂植入法，观察到皮炎芽生菌从菌丝相转变为酵母相的过程。首先，在室温下制备该真菌的玻片培养物。确认菌丝生长良好后，移除盖玻片，并切出一块边长约3毫米的方形培养基。小鼠剖腹后，将切下的每个琼脂块植入小鼠腹腔。植入琼脂块的小鼠每天处死两只，持续14天，之后每隔一周处死两只，持续2个月。因此，所有植入的琼脂块都被回收。直接用光学显微镜检查，并同时进行组织病理学和电子显微镜检查。在小鼠腹腔内，菌丝形成间生和顶生厚垣孢子。这些厚垣孢子随后膨胀成为酵母样细胞，并通过出芽进行增殖。
