Zhang Xiaojiao, Chen Hengying, Wang Yiyuan, Xu Qiujin, Qiu Xinzu, Cheng Li, Xiao Qizhi, Liu Yanhong, Zhang Jianduan, Zhang Hongzhong, Wu Hongyuan
Department of Child Health Care, Zhuhai Center for Maternal and Child Health Care, Zhuhai, Guangdong, China.
Department of Maternal and Child Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
BMC Pediatr. 2025 Mar 20;25(1):220. doi: 10.1186/s12887-025-05578-9.
Altered gut microbiota (GM) profiles have been documented in children with food allergies (FA) and experiencing malnutrition. This study explored the GM composition in children with FA across varying degrees of malnutrition including those without malnutrition and those with different severity levels.
Fresh faecal samples were collected from 120 children aged 1-6 years, including 40 FA children with adequate weight (FANM), 40 FA children with malnutrition (FAM), and 40 healthy controls. The hypervariable region of the 16 S rDNA gene was subsequently sequenced to assess bacterial communities.
Compared with healthy controls, the FANM group displayed a greater increase in the alpha diversity index. The FAM group exhibited an increase in seven genera, including Alistipes and Parabacteroides, compared to the control group, whereas nine genera were enriched in the FANM group. An analysis of clinical characteristics revealed a positive correlation between the relative abundance of the genus Faecalibacterium and the total IgE level. Fourteen pivotal microbial markers demonstrated substantial classification potential (AUC: 89.86%, 95% CI: 76.40-99.73% for FAM; AUC: 88.92%, 95% CI: 73.58-99.65% for FANM).
FA children exhibit distinct GM profiles depending on the presence of malnutrition, which suggests the need for tailored microbiota-targeted therapies.
食物过敏（FA）和营养不良儿童的肠道微生物群（GM）谱已被记录改变。本研究探讨了不同程度营养不良的FA儿童的GM组成，包括无营养不良的儿童和不同严重程度的儿童。
收集120名1 - 6岁儿童的新鲜粪便样本，包括40名体重正常的FA儿童（FANM）、40名营养不良的FA儿童（FAM）和40名健康对照。随后对16S rDNA基因的高变区进行测序以评估细菌群落。
与健康对照相比，FANM组的α多样性指数增加更大。与对照组相比，FAM组有7个属增加，包括阿里斯杆菌属和副拟杆菌属，而FANM组有9个属富集。临床特征分析显示，粪杆菌属的相对丰度与总IgE水平呈正相关。14个关键微生物标志物显示出显著的分类潜力（FAM组：AUC为89.86%，95%CI为76.40 - 99.73%；FANM组：AUC为88.92%，95%CI为73.58 - 99.65%）。
FA儿童根据是否存在营养不良表现出不同的GM谱，这表明需要针对微生物群进行个性化治疗。
