Madhusoodanan Karthika, Mooventhan Hemalatha, Mohanan Maneesha, Vaithilingam Mohanasrinivasan, Chandrasekaran Subathra Devi
Department of Biotechnology, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu 632014 India.
J Food Sci Technol. 2025 Apr;62(4):697-705. doi: 10.1007/s13197-024-06059-0. Epub 2024 Aug 23.
Menaquinone-7 has gained popularity due to its beneficial effects on health, there is an increased demand for its production among various sectors. In this study, vitamin MK-7 was produced from isolated from fermented (soy bean). This study includes isolation, screening of bacteria capable of producing Menaquinone - 7 [MK-7] and production of MK-7 was optimized using one factor optimization method. Different food samples including both fermented and non-fermented ones were collected from Vellore district and a total of 30 strains were isolated for the study. All of these were subjected for the screening of MK-7 using HPLC. This quantitative analysis determined that the bacterial strain KMV07, which was isolated from fermented soybean was identified as the producer of MK-7. Among the isolated strains, KMV07 showed a significant potency in MK-7 production with a yield of 16.74 ± 1.21 mg/L. Based on the morphology, biochemical and molecular identification, the strain was identified as (KMV07). The objective of the research was to increase the production of MK-7 by the strain KMV07. Using different parameters, one factor optimization was done in-order to increase the production. This study indicated that maximum production was achieved under culture conditions when maltose and tryptone were used as carbon and nitrogen sources, with a pH of 8, an inoculum size of 3 mL (8.25 × 10 CFU/mL) and an incubation time of 120th h. This study implies that (KMV07) isolated from could be a suitable candidate for the increased production of MK-7.
甲萘醌-7因其对健康的有益作用而受到欢迎，各行业对其生产的需求不断增加。在本研究中，维生素MK-7是从发酵（大豆）中分离出来的。本研究包括分离、筛选能够产生甲萘醌-7[MK-7]的细菌，并使用单因素优化方法对MK-7的生产进行优化。从韦洛尔地区收集了包括发酵和未发酵的不同食品样本，共分离出30株菌株用于研究。所有这些菌株都通过高效液相色谱法进行MK-7筛选。该定量分析确定，从发酵大豆中分离出的细菌菌株KMV07被鉴定为MK-7的生产者。在分离出的菌株中，KMV07在MK-7生产中表现出显著的效力，产量为16.74±1.21mg/L。根据形态学、生化和分子鉴定，该菌株被鉴定为（KMV07）。该研究的目的是提高菌株KMV07的MK-7产量。使用不同参数进行单因素优化以提高产量。本研究表明，当使用麦芽糖和胰蛋白胨作为碳源和氮源，pH值为8，接种量为3mL（8.25×10CFU/mL），培养时间为第120小时时，在培养条件下可实现最大产量。本研究表明，从[未提及具体物质]中分离出的（KMV07）可能是提高MK-7产量的合适候选菌株。
