使用纳米孔测序进行人乳头瘤病毒整合分析及其与宫颈癌前病变的关联
HPV integration profiling using nanopore sequencing and association with cervical precancerous lesion.
作者信息
Hou Ying, Ni Shoufeng, Liu Xin, Liu Xingyu, Wang Nan, Xu Fuqiang, Gao Jianyong, Li Yanmei, Zhou Yuxiang, Tang Huadong, Bian Meina, Li Xiulan, Zhang Lili, Wang Weiwei, Liu Qing
机构信息
Department of Gynecology, Beijing Youan Hospital, Capital Medical University, Beijing, China.
Geneis Beijing Co., Ltd., Beijing, China.
出版信息
Front Microbiol. 2025 Mar 5;16:1522550. doi: 10.3389/fmicb.2025.1522550. eCollection 2025.
OBJECTIVES
HPV infection and HPV DNA integration can lead to cervical cancer, but the relationship with lesion severity is unclear. This study aimed to investigate the correlation between HPV integration profile and cervical lesion extent.
MATERIALS AND METHODS
Twenty patients representing cervicitis, CIN I, CIN II, and CIN III underwent nanopore sequencing for HPV genotype and integration site analysis. HPV integration profiles were correlated with lesion severity. Gene Ontology (GO) and KEGG analysis were used to identify stage-specific genes and pathways.
RESULTS
HPV integration rates were 60, 60, 100, and 100% for cervicitis, CIN I, CIN II, and CIN III, respectively, with varying numbers of integrated genes. Each group had specific stage-related genes, with 83 shared genes linked to neuron development and cell-cell processes. CIN II and CIN III displayed more cancer-related pathway enrichment than earlier stages.
CONCLUSION
A positive correlation exists between HPV integration frequency and cervical lesion stage. Late-stage lesions showed heightened enrichment in cancer-related pathways through specific HPV-integrated genes.
目的
人乳头瘤病毒（HPV）感染和HPV DNA整合可导致宫颈癌，但其与病变严重程度的关系尚不清楚。本研究旨在探讨HPV整合谱与宫颈病变程度之间的相关性。
材料与方法
选取20例分别患有宫颈炎、CIN I、CIN II和CIN III的患者，对其进行HPV基因型和整合位点分析的纳米孔测序。将HPV整合谱与病变严重程度进行关联分析。采用基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析来鉴定阶段特异性基因和通路。
结果
宫颈炎、CIN I、CIN II和CIN III的HPV整合率分别为60%、60%、100%和100%，整合基因数量各不相同。每组都有特定的阶段相关基因，其中83个共享基因与神经元发育和细胞间过程相关。与早期阶段相比，CIN II和CIN III显示出更多与癌症相关的通路富集。
结论
HPV整合频率与宫颈病变阶段呈正相关。晚期病变通过特定的HPV整合基因在癌症相关通路中表现出更高的富集。
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