Rittisak Sriwiang, Charoen Ratchanee, Seamsin Saowalak, Buppha Jamin, Ruangthong Thanida, Muenkhling Sarinya, Riansa-Ngawong Wiboon, Eadmusik Sunee, Phattayakorn Kriangkrai, Jantrasee Sakwiboon, Savedboworn Wanticha
Department of Innovation and Product Development Technology, Faculty of Digital Agro-Industry, King Mongkut's University of Technology North Bangkok, Prachinburi, Thailand.
Department of Agro-Industry Technology and Management, Faculty of Digital Agro-Industry, King Mongkut's University of Technology North Bangkok, Prachinburi, Thailand.
Food Sci Biotechnol. 2024 Dec 18;34(6):1383-1399. doi: 10.1007/s10068-024-01794-8. eCollection 2025 Apr.
The purpose of this study was to assess the influence of collagen protein and various prebiotics as encapsulant materials, as well as double-layer coatings composed of chitosan or sericin, on the viability of encapsulated probiotic TISTR 2075. The storage stability of encapsulated probiotic was evaluated throughout 4 and 30 °C for 105 and 63 days, respectively. The log-linear inactivation rate () models indicated that co-encapsulated with alginate-collagen-inulin and double-layer coated with chitosan (ACI-C) had the lowest value. Furthermore, ACI-C demonstrated the greatest value in terms of the time required for the first decimal decrease () applied by Weibull non-linear model during storage at 4 and 30 °C. Encapsulated probiotic beads with ACI-C also exhibited the highest survivability after exposure to simulated gastrointestinal tract tolerance, heat resistance, and the degree hydrophobicity. The viability of cells significantly enhanced through coating with chitosan rather than sericin when employing the identical encapsulating agent.
The online version contains supplementary material available at 10.1007/s10068-024-01794-8.
本研究的目的是评估胶原蛋白和各种益生元作为包封材料，以及由壳聚糖或丝胶蛋白组成的双层涂层对包封益生菌TISTR 2075活力的影响。分别在4℃和30℃下对包封益生菌进行105天和63天的储存稳定性评估。对数线性失活率（）模型表明，与藻酸盐-胶原蛋白-菊粉共包封并双层涂覆壳聚糖（ACI-C）的 值最低。此外，在4℃和30℃储存期间，根据威布尔非线性模型，ACI-C在首次十进制减少（）所需时间方面表现出最大的值。具有ACI-C的包封益生菌珠在暴露于模拟胃肠道耐受性、耐热性和疏水性程度后也表现出最高的存活率。当使用相同的包封剂时，通过壳聚糖而非丝胶蛋白包被可显著提高细胞活力。
在线版本包含可在10.1007/s10068-024-01794-8获取的补充材料。
