Brenner S H, Lessing J B, D'Eletto R D, Weiss G
Obstet Gynecol. 1985 Jul;66(1):46-9.
The purpose of this study was to determine whether or not the immunoreactive relaxin contained in human pregnancy serum is biologically active. Relaxin was extracted from pooled serum of women in the third trimester of pregnancy. The extraction also was performed on pooled male serum and pooled serum from women in the follicular phase of the menstrual cycle. The extraction process began with delipidation of the serum followed by extraction of relaxin using 0.1% HCl per 50% acetone. Dialysis then was performed to remove low molecular weight substances (less than 3500 daltons). The partially purified extracts were applied individually to a muscle segment in the in vitro rat uterine horn bioassay for relaxin. The extracts of both male serum and female follicular-phase serum, containing no immunoreactive relaxin, resulted in tetanic contractions of the muscle segment. The extract of pregnancy serum, containing immunoreactive relaxin, produced inhibition of rat uterine horn contractility. This represents the first demonstration of relaxinlike bioactivity in human serum.
本研究的目的是确定人妊娠血清中所含的免疫反应性松弛素是否具有生物活性。从妊娠晚期妇女的混合血清中提取松弛素。同时也对男性混合血清和月经周期卵泡期妇女的混合血清进行了提取。提取过程首先是对血清进行脱脂，然后用每50%丙酮含0.1%盐酸的溶液提取松弛素。接着进行透析以去除低分子量物质（小于3500道尔顿）。将部分纯化的提取物分别应用于体外大鼠子宫角松弛素生物测定中的肌肉段。男性血清和女性卵泡期血清的提取物均不含免疫反应性松弛素，导致肌肉段强直性收缩。含有免疫反应性松弛素的妊娠血清提取物则抑制了大鼠子宫角的收缩力。这是首次在人血清中证明有类似松弛素的生物活性。
