Biochemical characterization of substances with myometrium contraction inhibitory action, originated from ovaries of pregnant sows.

In order to understand life itself, we need biotechnology to purify biologically active substances as well. One of these processes results peptides with myometrium contraction inhibitory effect, originated from the ovaries of pregnant sows, but different from relaxin. After collection and acidic-acetone extraction of the ovaries (product generation, primary purification) the substance with myometrium contraction inhibitory effect was further purified on Sephadex G-25 (fine), CM-52 and again Sephadex G-25 (fine) gels (secondary purification) without loosing the bioactivity of the substance (quality control). Product analysis by analytical high performance liquid chromatography and capillary isotachophoresis were performed at this stage of the experiments. The analysis mentioned above resulted in 2-15 different subfractions in the studied extract. This shows that the biologically active ovarian extract needs further purification before the planned final amino-acid analysis is performed.