Guoth J, Gróf J, Menyhárt J
Acta Physiol Hung. 1985;65(3):373-8.
In order to understand life itself, we need biotechnology to purify biologically active substances as well. One of these processes results peptides with myometrium contraction inhibitory effect, originated from the ovaries of pregnant sows, but different from relaxin. After collection and acidic-acetone extraction of the ovaries (product generation, primary purification) the substance with myometrium contraction inhibitory effect was further purified on Sephadex G-25 (fine), CM-52 and again Sephadex G-25 (fine) gels (secondary purification) without loosing the bioactivity of the substance (quality control). Product analysis by analytical high performance liquid chromatography and capillary isotachophoresis were performed at this stage of the experiments. The analysis mentioned above resulted in 2-15 different subfractions in the studied extract. This shows that the biologically active ovarian extract needs further purification before the planned final amino-acid analysis is performed.
为了理解生命本身，我们也需要生物技术来纯化生物活性物质。其中一个过程产生了具有抑制子宫肌层收缩作用的肽，它源自怀孕母猪的卵巢，但与松弛素不同。在收集卵巢并进行酸性丙酮提取（产物生成、初步纯化）后，具有抑制子宫肌层收缩作用的物质在葡聚糖凝胶G - 25（细）、CM - 52以及再次使用葡聚糖凝胶G - 25（细）凝胶上进一步纯化（二次纯化），同时不会损失该物质的生物活性（质量控制）。在实验的这个阶段，通过分析型高效液相色谱和毛细管等速电泳进行产物分析。上述分析在研究提取物中产生了2 - 15个不同的亚组分。这表明在进行计划中的最终氨基酸分析之前，生物活性卵巢提取物需要进一步纯化。
