Wang Zhixiong, Shang Xiankun, Wu Zixuan, Wei Jili, Tian Xiaoli, Zhang Guohui
College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China.
Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Sugarcane Genetic Improvement/Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
J Agric Food Chem. 2025 Apr 2;73(13):7669-7684. doi: 10.1021/acs.jafc.4c13219. Epub 2025 Mar 20.
is the key pest of sugar cane. Due to the difficulty associated with pesticide application in middle and late growth stages of sugar cane, the use of odorant attractants is a beneficial alternative to pesticides. Odorant-binding proteins (OBPs) in the insect olfactory system represent excellent targets for screening odorant attractants. Here, we identified 12 from the head transcriptome of adults, with four (/2/5/12) highly enriched in adult antennae. Ligand-binding assays for the four CsacOBPs showed that they can bind with 10 of the 30 host volatiles tested. Behavioral assays revealed that (+)-cedrol and 1-hexadecanol from the 10 compounds can attract the moths of both sexes. Protein-ligand interaction analyses identified five key amino acid residues involved in CsacOBPs' binding to (+)-cedrol and 1-hexadecanol. These findings have enhanced our molecular understanding of the host plant selection in and have facilitated the development of attractants for .
是甘蔗的主要害虫。由于在甘蔗生长中后期施用农药存在困难，使用气味引诱剂是替代农药的有益选择。昆虫嗅觉系统中的气味结合蛋白（OBP）是筛选气味引诱剂的理想靶点。在此，我们从成虫头部转录组中鉴定出12种，其中4种（/2/5/12）在成虫触角中高度富集。对这4种CsacOBP进行配体结合试验表明，它们能与所测试的30种寄主挥发物中的10种结合。行为试验表明，这10种化合物中的（+）-雪松醇和十六醇能吸引雌雄成虫。蛋白质-配体相互作用分析确定了CsacOBP与（+）-雪松醇和十六醇结合所涉及的5个关键氨基酸残基。这些发现加深了我们对寄主植物选择的分子理解，并促进了引诱剂的开发。
