Jia Zhifei, Qin Zeyu, Ge Xiaoyu, Xu Yongyu, Chen Zhenzhen
State Key Laboratory of Wheat Improvement, College of Plant Protection, Shandong Agricultural University, Tai'an 271000, China.
Int J Mol Sci. 2025 Sep 9;26(18):8784. doi: 10.3390/ijms26188784.
, an invasive pest native to Southeast Asia, exhibits rapid dispersal capacity and high eradication resistance. In recent years, there have been continuous records of its invasion into new host plants. Odorant-binding proteins (OBPs) are essential at the peripheral level of olfaction, and their olfactory function has been partially confirmed by research. This study explores the functions of key OBPs mediating host selection by measuring the in vivo and in vitro binding capabilities of OBPs from to host volatiles. Under exposure to more than five host volatiles, the two , and , exhibited significant differential transcriptional regulation. AspiOBP1 exhibited good binding affinity to ()-3-hexenol and 3-carene, and with binding energies greater than -3 kcal/mol, ARG-79 might be the critical amino acid site for AspiOBP1 binding to host volatiles. AspiOBP2 exhibited no binding to any of the six tested volatiles in fluorescent competitive binding assays. Adults fed with showed significantly reduced behavioral and EAG responses to the attractant 3-carene and two repellents [()-3-hexenol and nonanal]. Adults fed with lost both behavioral and EAG responses to the attractant 3-carene and the repellent ()-3-hexenol. The findings of this study not only elucidate the binding mechanisms between OBPs of and host volatiles but also provide new targets for the future development of novel plant-derived insecticides and RNA-based pesticides to control this pest.
[害虫名称]是一种原产于东南亚的入侵性害虫，具有快速扩散能力和高抗除能力。近年来，不断有其入侵新寄主植物的记录。气味结合蛋白（OBPs）在嗅觉外周水平至关重要，其嗅觉功能已通过研究得到部分证实。本研究通过测量[害虫名称]的OBPs与寄主挥发物的体内和体外结合能力，探索介导寄主选择的关键OBPs的功能。在暴露于五种以上寄主挥发物时，两种[OBP名称1]和[OBP名称2]表现出显著的差异转录调控。AspiOBP1对（）-3-己烯醇和3-蒈烯表现出良好的结合亲和力，结合能大于-3 kcal/mol，ARG-79可能是AspiOBP1与寄主挥发物结合的关键氨基酸位点。在荧光竞争结合试验中，AspiOBP2对六种测试挥发物中的任何一种都没有结合。用[处理物质1]喂养的成虫对引诱剂3-蒈烯和两种驱避剂[（）-3-己烯醇和壬醛]的行为和EAG反应显著降低。用[处理物质2]喂养的成虫对引诱剂3-蒈烯和驱避剂（）-3-己烯醇失去了行为和EAG反应。本研究结果不仅阐明了[害虫名称]的OBPs与寄主挥发物之间的结合机制，还为未来开发新型植物源杀虫剂和基于RNA的杀虫剂以控制这种害虫提供了新的靶点。
