Bitiņa-Barlote Ērika, Plonis Juris, Andrejeva Margarita, Vjaters Egils, Gardovskis Jānis, Daneberga Zanda, Miklaševičs Edvīns, Nakazawa-Miklaševiča Miki
Institute of Oncology and Molecular Genetics, Rīga Stradiņš University, Latvia.
Pauls Stradiņš Clinical University Hospital, Centre of Urology, Riga, Latvia.
Cent European J Urol. 2024;77(3):361-374. doi: 10.5173/ceju.2023.279.R1. Epub 2024 Sep 30.
Exosomes contain nucleic acids and proteins inside of them. These are suggested as cell-cell communication materials and it is considered that they can modulate the status of other cells.
To understand the bladder cancer (BC) related exosomal microRNAs (miRNAs), we compared the 752 urine exosomal miRNAs in healthy control (n = 7), low grade (LG) BC (n = 6) and high grade (HG) BC (n = 6) by RT-qPCR.
The differential expressing (DE) urine exosomal miRNAs (2 > fold regulation) were 96 and 78 in LG and HG, respectively. Our exosomal miRNAs profiles cover many miRNAs which have been reported in BC patients' tissues and other biofluids. Most DE exosomal miRNAs were up-regulated in the profiles. Seven up-regulated exosomal miRNAs in the LG group (miR-28-5p, miR-16-5p, miR-28-3p, miR-24-3p, miR-25-3p, miR-19b-3p and miR10b-5p) and 3 miRNAs in the HG group (miR-150-5p, miR-28-5p and miR28-3p) were found as directly targeting. Twenty-two and 18 targeting miRNAs were observed in up-regulated miRNAs of LG and HG. The target genes of these exosomal miRNAs and their interaction network predicted that the is the strongest hub gene in both BC groups exosomal miRNA networks. Several DE miRNAs were found that could potentially be used as biomarkers for the diagnosis of BC.
Profiles of urinal exosomal miRNAs derived from BC manifested potentially epigenetic regulation of the and genes as compared to other oncogenes and tumour suppressors.
外泌体内部含有核酸和蛋白质。这些被认为是细胞间通讯物质，并且人们认为它们可以调节其他细胞的状态。
为了解膀胱癌（BC）相关的外泌体微小RNA（miRNA），我们通过逆转录定量聚合酶链反应（RT-qPCR）比较了健康对照者（n = 7）、低级别（LG）膀胱癌患者（n = 6）和高级别（HG）膀胱癌患者（n = 6）的752种尿液外泌体miRNA。
LG组和HG组中差异表达（DE）的尿液外泌体miRNA（调控倍数>2）分别为96种和78种。我们的外泌体miRNA谱涵盖了许多在BC患者组织和其他生物流体中已报道的miRNA。大多数DE外泌体miRNA在谱中呈上调状态。LG组中有7种上调的外泌体miRNA（miR-28-5p、miR-16-5p、miR-28-3p、miR-24-3p、miR-25-3p、miR-19b-3p和miR10b-5p）以及HG组中的3种miRNA（miR-150-5p、miR-28-5p和miR28-3p）被发现为直接靶向。在LG组和HG组上调的miRNA中分别观察到22种和18种靶向miRNA。这些外泌体miRNA的靶基因及其相互作用网络预测，[此处原文缺失具体基因名称]是两个BC组外泌体miRNA网络中最强的枢纽基因。发现几种DE miRNA可能潜在地用作BC诊断的生物标志物。
与其他癌基因和肿瘤抑制基因相比，源自BC的尿液外泌体miRNA谱显示出对[此处原文缺失具体基因名称]和[此处原文缺失具体基因名称]基因的潜在表观遗传调控。
