用于生物生产有用化合物的人工代谢途径的设计与构建。
Design and construction of artificial metabolic pathways for the bioproduction of useful compounds.
作者信息
Shirai Tomokazu
机构信息
RIKEN Center for Sustainable Resource Science, Cell Factory Research Team, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
出版信息
Plant Biotechnol (Tokyo). 2024 Sep 25;41(3):261-266. doi: 10.5511/plantbiotechnology.24.0721c.
To efficiently produce useful compounds using biological cells, it is essential to optimally design all metabolic reactions and pathways, including not only the flow of carbon within the cell but also the production and consumption of energy and the balance of oxidation-reduction. Computational scientific methods are effective for the rational design of metabolic pathways and the optimization of metabolic fluxes. Based on this blueprint, it is crucial to accurately construct the cell, test and analyze whether it conforms to the design, and learn from the results to redesign the system in an effective cycle. This review introduces essential metabolic design techniques in synthetic biology and discusses the potential of using plant cells or plant genes effectively in synthetic biology for the production of useful compounds.
为了利用生物细胞高效生产有用的化合物，优化设计所有代谢反应和途径至关重要，这不仅包括细胞内碳的流动，还包括能量的产生和消耗以及氧化还原平衡。计算科学方法对于合理设计代谢途径和优化代谢通量是有效的。基于此蓝图，准确构建细胞、测试和分析其是否符合设计，并从结果中学习以在有效循环中重新设计系统至关重要。本文综述介绍了合成生物学中的基本代谢设计技术，并讨论了在合成生物学中有效利用植物细胞或植物基因生产有用化合物的潜力。
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