Department of Cardiology, Aarhus University Hospital, Aarhus, Denmark.
Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.
Physiol Rep. 2024 Jul;12(13):e16132. doi: 10.14814/phy2.16132.
Different rat strains are used in various animal models of pulmonary hypertension and right ventricular (RV) failure. No systematic assessment has been made to test differences in RV response to pressure overload between rat strains. We compared RV adaptation to pulmonary trunk banding (PTB) in Wistar (W), Sprague Dawley (SD), and Fischer344 (F) rats by hemodynamic profiling focusing on diastolic function. Age-matched male rat weanlings were randomized to sham surgery (W-sham, n = 5; SD-sham, n = 4; F-sham, n = 4) or PTB (W-PTB, n = 8; SD-PTB, n = 8; F-PTB, n = 8). RV function was evaluated after 5 weeks by echocardiography, cardiac MRI, and invasive pressure-volume measurements. PTB caused RV failure and increased RV systolic pressures four-fold in all three PTB groups compared with sham. W- and SD-PTB had a 2.4-fold increase in RV end-systolic volume index compared with sham, while F-PTB rats were less affected. Diastolic and right atrial impairment were evident by increased RV end-diastolic elastance, filling pressure, and E/e' in PTB rats compared with sham, again F-PTB the least affected. In conclusions, PTB caused RV failure with signs of diastolic dysfunction. Despite a similar increase in RV systolic pressure, F-PTB rats showed less RV dilatation and a more preserved diastolic function compared with W- and SD-PTB.
不同的大鼠品系被用于各种肺动脉高压和右心室（RV）衰竭的动物模型中。目前还没有系统的评估来测试大鼠品系之间对压力超负荷的 RV 反应的差异。我们通过血流动力学分析比较了 Wistar（W）、Sprague Dawley（SD）和 Fischer344（F）大鼠在肺动脉干结扎（PTB）后的 RV 适应性，重点关注舒张功能。将同龄雄性幼鼠随机分为假手术组（W-sham，n=5；SD-sham，n=4；F-sham，n=4）或 PTB 组（W-PTB，n=8；SD-PTB，n=8；F-PTB，n=8）。在 5 周后，通过超声心动图、心脏 MRI 和侵入性压力-容积测量评估 RV 功能。PTB 导致所有三组 PTB 大鼠的 RV 衰竭和 RV 收缩压增加四倍，与假手术组相比。与假手术组相比，W-和 SD-PTB 的 RV 收缩末期容积指数增加了 2.4 倍，而 F-PTB 大鼠受影响较小。与假手术组相比，PTB 大鼠的 RV 舒张末期弹性、充盈压和 E/e'增加，表明存在 RV 舒张和右心房功能障碍，而 F-PTB 大鼠受影响最小。总之，PTB 导致 RV 衰竭，并伴有舒张功能障碍的迹象。尽管 RV 收缩压有相似的增加，但与 W-和 SD-PTB 相比，F-PTB 大鼠的 RV 扩张程度较小，舒张功能保存较好。
